Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …

Fundamental properties of light unavoidably impose features on images collected using
fluorescence microscopes. Accounting for these features is often critical in quantitatively …

Imaging of both the positions and orientations of single fluorophores, termed single-
molecule orientation-localization microscopy, is a powerful tool for the study of biochemical …

Synthetic peptides that self-assemble into cross-β fibrils are versatile building blocks for
engineered biomaterials due to their modularity and biocompatibility, but their structural and …

Many biophysical techniques, such as single-molecule fluorescence correlation
spectroscopy, Förster resonance energy transfer, and fluorescence anisotropy, measure …

Current methods for single-molecule orientation localization microscopy (SMOLM) require
optical setups and algorithms that can be prohibitively slow and complex, limiting …

Abstract Point Spread Function (PSF) engineering is an effective method to increase the
sensitivity of single-molecule fluorescence images to specific parameters. Classical phase …

This tutorial provides an overview of the local description of polarization for nonparaxial
light, for which all Cartesian components of the electric field are significant. The polarization …

Localization-based super-resolution imaging techniques have improved the spatial
resolution of optical microscopy well below the diffraction limit, yet encoding additional …

Single-molecule microscopy is advantageous in characterizing heterogeneous dynamics at
the molecular level. However, there are several challenges that currently hinder the wide …