BACKGROUND Biomolecules can serve as natural labels for microscopy by measuring their
molecular vibration spectra in living cells and tissues. However, the transition from …

Coherent Raman scattering (CRS) microscopy is a chemical imaging modality that provides
contrast based on intrinsic biomolecular vibrations. To date, endeavors on instrumentation …

Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free
visualization and analysis of functional, endogenous biomolecules in living systems. When …

Spontaneous Raman (SR) microscopy allows label‐free chemically specific imaging based
on the vibrational response of molecules; however, due to the low Raman scattering cross …

First, the potential role of Raman-based techniques in biomedicine is introduced. Second,
an overview about the instrumentation for spontaneous and coherent Raman scattering …

Conspectus Traditionally, molecules are analyzed in a test tube. Taking biochemistry as an
example, the majority of our knowledge about cellular content comes from analysis of fixed …

Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging nonlinear
vibrational imaging technique that delivers label-free chemical maps of cells and tissues. In …

Chemical imaging offers critical information to understand the fundamentals in biology and
to assist clinical diagnostics. Label-free chemical imaging piques a general interest since it …

Stimulated Raman scattering (SRS) microscopy is an emerging platform for vibrational
imaging of living systems. We present microsecond-scale SRS spectroscopic imaging by …

In vivo vibrational spectroscopic imaging is inhibited by relatively slow spectral acquisition
on the second scale and low photon collection efficiency for a highly scattering system …