Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae

GR Navale, MS Dharne, SS Shinde - Applied Microbiology and …, 2021‏ - Springer
Isoprenoids, often called terpenoids, are the most abundant and highly diverse family of
natural organic compounds. In plants, they play a distinct role in the form of photosynthetic …

Engineering terpene synthases and their substrates for the biocatalytic production of terpene natural products and analogues

LA Johnson, RK Allemann - Chemical Communications, 2025‏ - pubs.rsc.org
Terpene synthases produce a wide number of hydrocarbon skeletons by controlling
intramolecular rearrangements of allylic pyrophosphate subtrates via reactive carbocation …

Redesigning the molecular choreography to prevent hydroxylation in germacradien-11-ol synthase catalysis

PL Srivastava, AM Escorcia, F Huynh, DJ Miller… - ACS …, 2021‏ - ACS Publications
Natural sesquiterpene synthases have evolved to make complex terpenoids by quenching
reactive carbocations either by proton transfer or by hydroxylation (water capture) …

Nucleophilic Water Capture or Proton Loss: Single Amino Acid Switch Converts δ‐Cadinene Synthase into Germacradien‐4‐ol Synthase

M Loizzi, V González, DJ Miller, RK Allemann - ChemBioChem, 2018‏ - Wiley Online Library
Abstract δ‐Cadinene synthase is a sesquiterpene cyclase that utilises the universal achiral
precursor farnesyl diphosphate (FDP) to generate predominantly the bicyclic sesquiterpene …

Enhancing epi‐cedrol production in Escherichia coli by fusion expression of farnesyl pyrophosphate synthase and epi‐cedrol synthase

GR Navale, P Sharma, MS Said… - Engineering in Life …, 2019‏ - Wiley Online Library
Terpene synthase catalyses acyclic diphosphate farnesyl diphosphate into desired
sesquiterpenes. In this study, a fusion enzyme was constructed by linking Santalum album …

[كتاب][B] Organic Reaction Mechanisms: 2013

AC Knipe, MG Moloney - 2016‏ - Wiley Online Library
The present volume, the forty-ninth in the series, surveys research on organic reaction
mechanisms described in the available literature dated 2013. In order to limit the size of the …

Retracted Article: Synthesis of deuterated isopentyl pyrophosphates for chemo-enzymatic labelling methods: GC-EI-MS based 1, 2-hydride shift in epicedrol …

MS Said, GR Navale, JM Gajbhiye, SS Shinde - RSC advances, 2019‏ - pubs.rsc.org
A sesquiterpene epicedrol cyclase mechanism was elucidated based on the gas
chromatography coupled to electron impact mass spectrometry fragmentation data of …

Carbocations

DA Klumpp - Organic Reaction Mechanisms· 2013: An annual …, 2016‏ - Wiley Online Library
This chapter reviews the use of carbocations in synthetic chemistry. This includes surveys of
new methods of generating carbocations, evaluation of their reactivities, and their use in …

Improved production of epi-cedrol and santalene by fusion protein expression: Stability study and cyclization mechanism of epi-cedrol biosynthesis

GR Navale - 2021‏ - dspace.ncl.res.in
A wide range of secondary metabolites are produced by living organisms such as plants,
bacteria and fungi as a part of their defense system against herbivores, pests and pathogens …