The evolutionary and functional significance of germline immunoglobulin gene variation

M Pennell, OL Rodriguez, CT Watson, V Greiff - Trends in immunology, 2023 - cell.com
The recombination between immunoglobulin (IG) gene segments determines an individual's
naïve antibody repertoire and, consequently,(auto) antigen recognition. Emerging evidence …

Idiosyncratic epistasis leads to global fitness–correlated trends

CW Bakerlee, AN Nguyen Ba, Y Shulgina… - Science, 2022 - science.org
Epistasis can markedly affect evolutionary trajectories. In recent decades, protein-level
fitness landscapes have revealed extensive idiosyncratic epistasis among specific …

Co-evolution of interacting proteins through non-contacting and non-specific mutations

D Ding, AG Green, B Wang, TLV Lite… - Nature ecology & …, 2022 - nature.com
Proteins often accumulate neutral mutations that do not affect current functions but can
profoundly influence future mutational possibilities and functions. Understanding such …

Insights into next generation sequencing guided antibody selection strategies

MF Erasmus, F Ferrara, S D'Angelo, L Spector… - Scientific reports, 2023 - nature.com
Therapeutic antibody discovery often relies on in-vitro display methods to identify lead
candidates. Assessing selected output diversity traditionally involves random colony picking …

Binding affinity landscapes constrain the evolution of broadly neutralizing anti-influenza antibodies

AM Phillips, KR Lawrence, A Moulana, T Dupic… - Elife, 2021 - elifesciences.org
Over the past two decades, several broadly neutralizing antibodies (bnAbs) that confer
protection against diverse influenza strains have been isolated. Structural and biochemical …

Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site

BB Banach, S Pletnev, AS Olia, K Xu, B Zhang… - Nature …, 2023 - nature.com
The HIV-1 fusion peptide (FP) represents a promising vaccine target, but global FP
sequence diversity among circulating strains has limited anti-FP antibodies to~ 60 …

Large-scale antibody immune response map** of splenic B cells and bone marrow plasma cells in a transgenic mouse model

X Pan, SN López Acevedo, C Cuziol… - Frontiers in …, 2023 - frontiersin.org
Molecular characterization of antibody immunity and human antibody discovery is mainly
carried out using peripheral memory B cells, and occasionally plasmablasts, that express B …

An optimized thermodynamics integration protocol for identifying beneficial mutations in antibody design

Z Sheng, JS Bimela, M Wang, Z Li, Y Guo… - Frontiers in …, 2023 - frontiersin.org
Accurate identification of beneficial mutations is central to antibody design. Many knowledge-
based (KB) computational approaches have been developed to predict beneficial mutations …

Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron

IT Teng, AF Nazzari, M Choe, T Liu… - PloS one, 2022 - journals.plos.org
Since the outbreak of the COVID-19 pandemic, widespread infections have allowed SARS-
CoV-2 to evolve in human, leading to the emergence of multiple circulating variants. Some …

Map** monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens

MO de Souza, B Madan, IT Teng, A Huang… - Frontiers in …, 2022 - frontiersin.org
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged
continuously, challenging the effectiveness of vaccines, diagnostics, and treatments …