Structural biology of CRISPR–Cas immunity and genome editing enzymes

JY Wang, P Pausch, JA Doudna - Nature Reviews Microbiology, 2022 - nature.com
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …

Molecular mechanisms of CRISPR-Cas immunity in bacteria

PM Nussenzweig, LA Marraffini - Annual review of genetics, 2020 - annualreviews.org
Prokaryotes have developed numerous defense strategies to combat the constant threat
posed by the diverse genetic parasites that endanger them. Clustered regularly interspaced …

Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools

J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …

The application of DNA polymerases and Cas9 as representative of DNA-modifying enzymes group in DNA sensor design

J Dronina, US Bubniene, A Ramanavicius - Biosensors and Bioelectronics, 2021 - Elsevier
Rapid detection of nucleic acids (DNA or RNA) by inexpensive, selective, accurate, and
highly sensitive methods is very important for biosensors. DNA-sensors based on DNA …

Comprehensive classification of ABC ATPases and their functional radiation in nucleoprotein dynamics and biological conflict systems

A Krishnan, AM Burroughs, LM Iyer… - Nucleic acids …, 2020 - academic.oup.com
ABC ATPases form one of the largest clades of P-loop NTPase fold enzymes that catalyze
ATP-hydrolysis and utilize its free energy for a staggering range of functions from transport to …

Creating memories: molecular mechanisms of CRISPR adaptation

H Lee, DG Sashital - Trends in biochemical sciences, 2022 - cell.com
Prokaryotes use clustered regularly interspaced short palindromic repeat (CRISPR) and
CRISPR-associated (Cas) proteins as an adaptive immune system. CRISPR-Cas systems …

Disease-relevant β2-microglobulin variants share a common amyloid fold

M Wilkinson, RU Gallardo, RM Martinez… - Nature …, 2023 - nature.com
Abstract β2-microglobulin (β2m) and its truncated variant ΔΝ6 are co-deposited in amyloid
fibrils in the joints, causing the disorder dialysis-related amyloidosis (DRA). Point mutations …

A truncated anti-CRISPR protein prevents spacer acquisition but not interference

C Philippe, C Morency, PL Plante, E Zufferey… - Nature …, 2022 - nature.com
CRISPR-Cas systems in prokaryotic cells provide an adaptive immunity against invading
nucleic acids. For example, phage infection leads to addition of new immunity (spacer …

Advances and insights in the diagnosis of viral infections

J Dronina, U Samukaite-Bubniene… - Journal of …, 2021 - Springer
Viral infections are the most common among diseases that globally require around 60
percent of medical care. However, in the heat of the pandemic, there was a lack of medical …

[HTML][HTML] CRISPR/Cas9 based genome editing for targeted transcriptional control in triple-negative breast cancer

DD Singh, I Han, EH Choi, DK Yadav - Computational and structural …, 2021 - Elsevier
Breast cancer (BC) is the most common type of cancer in women at the global level and the
highest mortality rate has been observed with triple-negative breast cancer (TNBC) …