Post-translational modifications modulate the activity of most eukaryote proteins. Analysis of
these modifications presents formidable challenges but their determination generates …

Proteomics is an increasingly powerful and indispensable technology in molecular cell
biology. It can be used to identify the components of small protein complexes and large …

Peptide sequencing is the basis of mass spectrometry–driven proteomics. Here we show
that in the linear ion trap–orbitrap mass spectrometer (LTQ Orbitrap) peptide ions can be …

▪ Abstract A decade after the discovery of electrospray and matrix-assisted laser desorption
ionization (MALDI), methods that finally allowed gentle ionization of large biomolecules …

A brief introduction is presented to the basic principles and application of a quadrupole–time‐
of‐flight (TOF) tandem mass spectrometer. The main features of reflecting TOF instruments …

Determining the site of a regulatory phosphorylation event is often essential for elucidating
specific kinase–substrate relationships, providing a handle for understanding essential …

Since its introduction, the orbitrap has proven to be a robust mass analyzer that can routinely
deliver high resolving power and mass accuracy. Unlike conventional ion traps such as the …

In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand
signaling processes, we must first acquire an inventory of phosphoproteins and their …

Hybrid quadrupole time-of-flight (QTOF) mass spectrometry is one of the two major
principles used in proteomics. Although based on simple fundamentals, it has over the last …

Reversible phosphorylation is a key event in many biological processes and is therefore a
much studied phenomenon. The mass spectrometric (MS) analysis of phosphorylation is …