[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

[HTML][HTML] Genome engineering for crop improvement and future agriculture

C Gao - Cell, 2021 - cell.com
Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

An efficient CRISPR–Cas12a promoter editing system for crop improvement

J Zhou, G Liu, Y Zhao, R Zhang, X Tang, L Li, X Jia… - Nature Plants, 2023 - nature.com
Promoter editing represents an innovative approach to introduce quantitative trait variation
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …

Broad-spectrum resistance to bacterial blight in rice using genome editing

R Oliva, C Ji, G Atienza-Grande, JC Huguet-Tapia… - Nature …, 2019 - nature.com
Bacterial blight of rice is an important disease in Asia and Africa. The pathogen,
Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription …

CRISPR/Cas genome editing and precision plant breeding in agriculture

K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

OX Dong, S Yu, R Jain, N Zhang, PQ Duong… - Nature …, 2020 - nature.com
Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a
desirable alternative to insertions at random sites achieved through conventional methods …

Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9

X Ma, X Zhang, H Liu, Z Li - Nature Plants, 2020 - nature.com
Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant
science and hold enormous promise in crop improvement. Conventional transgene …

The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

[PDF][PDF] CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects

H Manghwar, B Li, X Ding, A Hussain… - Advanced …, 2020 - Wiley Online Library
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …

PAM-less plant genome editing using a CRISPR–SpRY toolbox

Q Ren, S Sretenovic, S Liu, X Tang, L Huang, Y He… - Nature plants, 2021 - nature.com
The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …