Combating antimicrobial resistance via single-cell diagnostic technologies powered by droplet microfluidics

K Hsieh, KE Mach, P Zhang, JC Liao… - Accounts of chemical …, 2021 - ACS Publications
Conspectus Antimicrobial resistance is a global threat that if left unchecked could lead to 10
million annual mortalities by 2050. One factor contributing to the rise of multi-drug-resistant …

[HTML][HTML] Advances in Host Depletion and Pathogen Enrichment Methods for Rapid Sequencing–Based Diagnosis of Bloodstream Infection

MSI Sajib, K Brunker, K Oravcova, P Everest… - The Journal of Molecular …, 2024 - Elsevier
Bloodstream infection is a major cause of morbidity and death worldwide. Timely and
appropriate treatment can reduce mortality among critically ill patients. Current diagnostic …

A Cascaded Droplet Microfluidic Platform Enables High‐Throughput Single Cell Antibiotic Susceptibility Testing at Scale

P Zhang, AM Kaushik, K Hsieh, S Li, S Lewis… - Small …, 2022 - Wiley Online Library
The global threat of antibiotic resistance underscores critical but unmet needs for rapid
antibiotic susceptibility testing (AST) technologies. To this end, droplet microfluidic‐based …

Direct single-cell antimicrobial susceptibility testing of Escherichia coli in urine using a ready-to-use 3D microwell array chip

W Wu, G Cai, Y Liu, Y Suo, B Zhang, W **, Y Yu… - Lab on a Chip, 2023 - pubs.rsc.org
Empirical antibiotic therapies are prescribed for treating uncomplicated urinary tract
infections (UTIs) due to the long turnaround time of conventional antimicrobial susceptibility …

Label-free 1D microfluidic dipstick counting of microbial colonies and bacteriophage plaques

Sİ Dönmez, SH Needs, HMI Osborn, NM Reis… - Lab on a Chip, 2022 - pubs.rsc.org
Counting viable bacterial cells and functional bacteriophage is fundamental to microbiology
underpinning research, surveillance, biopharmaceuticals and diagnostics. Colony forming …

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

AT Abafogi, T Wu, D Lee, J Lee, G Cho, LP Lee… - Journal of …, 2022 - Springer
Background Sepsis is caused mainly by infection in the blood with a broad range of bacterial
species. It can be diagnosed by molecular diagnostics once compounds in the blood that …

Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenic E. coli, mass-manufacturing and …

SH Needs, J Pivetal, J Hayward, SP Kidd… - Sensors & …, 2023 - pubs.rsc.org
Innovation in infection based point-of-care (PoC) diagnostics is vital to avoid unnecessary
use of antibiotics and the development of antimicrobial resistance. Several groups including …

[HTML][HTML] Combining deep learning and droplet microfluidics for rapid and label-free antimicrobial susceptibility testing of colistin

J Riti, G Sutra, T Naas, H Volland, S Simon… - Biosensors and …, 2024 - Elsevier
Efficient tools for rapid antibiotic susceptibility testing (AST) are crucial for appropriate use of
antibiotics, especially colistin, which is now often considered a last resort therapy with …

Miniaturised broth microdilution for simplified antibiotic susceptibility testing of Gram negative clinical isolates using microcapillary devices

SH Needs, N Saiprom, Z Rafaque, W Imtiaz… - Analyst, 2022 - pubs.rsc.org
Antibiotic resistance is a major global challenge. Although microfluidic antibiotic
susceptibility tests (AST) offer great potential for rapid and portable testing to inform correct …

Quantitative assessment of automated purification and concentration of E. coli bacteria

NSF Guzmán, MW Badawy, MA Stockslager, ML Farrell… - SLAS technology, 2023 - Elsevier
Automated methods for rapidly purifying and concentrating bacteria from environmental
interferents are needed in next-generation applications for anything from water purification …