Functional tools are needed to understand complex biological systems. Here we review how
chemical reporters in conjunction with bioorthogonal labeling methods can be used to …

Labelling of nucleic acids as biologically important cellular components is a crucial
prerequisite for the visualization and understanding of biological processes. Efficient …

Vinyl‐2′‐deoxyuridine (VdU) is the first reported metabolic probe for cellular DNA
synthesis that can be visualized by using an inverse electron demand Diels–Alder reaction …

Viral DNA trafficking in cells has large impacts on physiology and disease development.
Current methods lack the resolution and accuracy to visualize and quantify viral DNA …

Metabolic incorporation of azido nucleoside analogues into living cells can enable sensitive
detection of DNA replication through copper (I)‐catalyzed azide–alkyne cycloaddition …

The modification of RNA with fluorophores, affinity tags and reactive moieties is of enormous
utility for studying RNA localization, structure and dynamics as well as diverse biological …

Bioorthogonal chemistry has mainly been developed for proteins and carbohydrates. The
chemistry of nucleic acids is different, and bioorthogonal labeling strategies that were …

A complete series of 5-substituted uracil or cytosine, as well as 7-substituted 7-
deazaadenine and 7-deazaguanine 2′-deoxyribonucleoside triphosphates (dNTPs) …

Abstract A series of 7‐substituted 7‐deazaadenine and 5‐substituted cytosine 2′‐
deoxyribonucleoside triphosphates (dNTPs) were tested for their competitive incorporations …

Imaging technologies developed in the early 20th century achieved contrast solely by
relying on macroscopic and morphological differences between the tissues of interest and …