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An updated view on horseradish peroxidases: recombinant production and biotechnological applications
FW Krainer, A Glieder - Applied microbiology and biotechnology, 2015 - Springer
Horseradish peroxidase has been the subject of scientific research for centuries. It has been
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …
Production and purification of the multifunctional enzyme horseradish peroxidase
O Spadiut, C Herwig - Pharmaceutical bioprocessing, 2013 - pmc.ncbi.nlm.nih.gov
The oxidoreductase horseradish peroxidase (HRP) is used in numerous industrial and
medical applications. In this review, we briefly describe this well-studied enzyme and focus …
medical applications. In this review, we briefly describe this well-studied enzyme and focus …
Glycosylation increases active site rigidity leading to improved enzyme stability and turnover
Glycosylation is the most prevalent protein post‐translational modification, with a quarter of
glycosylated proteins having enzymatic properties. Yet, the full impact of glycosylation on the …
glycosylated proteins having enzymatic properties. Yet, the full impact of glycosylation on the …
[HTML][HTML] A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli
T Gundinger, O Spadiut - Journal of biotechnology, 2017 - Elsevier
Horseradish peroxidase (HRP) is used in various biotechnological and medical
applications. Since its isolation from plant provides several disadvantages, the bacterium …
applications. Since its isolation from plant provides several disadvantages, the bacterium …
[HTML][HTML] Scalable High-Performance Production of Recombinant Horseradish Peroxidase from E. coli Inclusion Bodies
D Humer, J Ebner, O Spadiut - International Journal of Molecular …, 2020 - mdpi.com
Horseradish peroxidase (HRP), an enzyme omnipresent in biotechnology, is still produced
from hairy root cultures, although this procedure is time-consuming and only gives low …
from hairy root cultures, although this procedure is time-consuming and only gives low …
A general approach to protein folding using thermostable exoshells
In vitro protein folding is a complex process which often results in protein aggregation, low
yields and low specific activity. Here we report the use of nanoscale exoshells (tES) to …
yields and low specific activity. Here we report the use of nanoscale exoshells (tES) to …
[HTML][HTML] Production strategies for active heme-containing peroxidases from E. coli inclusion bodies–a review
B Eggenreich, M Willim, DJ Wurm, C Herwig… - Biotechnology …, 2016 - Elsevier
Heme-containing peroxidases are frequently used in medical applications. However, these
enzymes are still extracted from their native source, which leads to inadequate yields and a …
enzymes are still extracted from their native source, which leads to inadequate yields and a …
[HTML][HTML] Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris
FW Krainer, MA Gerstmann, B Darnhofer… - Journal of …, 2016 - Elsevier
Horseradish peroxidase (HRP) is a high-demand enzyme for applications in diagnostics,
bioremediation, biocatalysis and medicine. Current HRP preparations are isolated from …
bioremediation, biocatalysis and medicine. Current HRP preparations are isolated from …
Recombinant horseradish peroxidase C1A immobilized on hydrogel matrix for dye decolorization and its mechanism on acid blue 129 decolorization
YJ Wang, KZ Xu, H Ma, XR Liao, G Guo, F Tian… - Applied Biochemistry …, 2020 - Springer
In this study, horseradish peroxidase C1A (HRP C1A) from Armoracia rusticana was
expressed in Escherichia coli as an inclusion body. Subsequently, an active recombinant …
expressed in Escherichia coli as an inclusion body. Subsequently, an active recombinant …
[HTML][HTML] Efficient in vitro refolding and functional characterization of recombinant human liver carboxylesterase (CES1) expressed in E. coli
U Boonyuen, K Promnares, S Junkree, NPJ Day… - Protein Expression and …, 2015 - Elsevier
Abstract Human liver carboxylesterase 1 (CES1) plays a critical role in the hydrolysis of
various ester-and amide-containing molecules, including active metabolites, drugs and …
various ester-and amide-containing molecules, including active metabolites, drugs and …