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Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
A primer to directed evolution: current methodologies and future directions
Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …
Methods for the directed evolution of proteins
MS Packer, DR Liu - Nature Reviews Genetics, 2015 - nature.com
Directed evolution has proved to be an effective strategy for improving or altering the activity
of biomolecules for industrial, research and therapeutic applications. The evolution of …
of biomolecules for industrial, research and therapeutic applications. The evolution of …
Modified nucleic acid aptamers: development, characterization, and biological applications
Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural
interactions. To improve the properties and performance of aptamers, modified nucleotides …
interactions. To improve the properties and performance of aptamers, modified nucleotides …
Taxonomy, physiology, and natural products of Actinobacteria
EA Barka, P Vatsa, L Sanchez… - Microbiology and …, 2016 - journals.asm.org
Actinobacteria are Gram-positive bacteria with high G+ C DNA content that constitute one of
the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and …
the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and …
Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes
C Engler, R Gruetzner, R Kandzia, S Marillonnet - PloS one, 2009 - journals.plos.org
We have developed a protocol to assemble in one step and one tube at least nine separate
DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained …
DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained …
Improving industrial yeast strains: exploiting natural and artificial diversity
Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …
Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum
Actinobacteria constitute one of the largest phyla among Bacteria and represent gram-
positive bacteria with a high G+ C content in their DNA. This bacterial group includes …
positive bacteria with a high G+ C content in their DNA. This bacterial group includes …
[КНИГА][B] Enzymes in industry: production and applications
W Aehle - 2007 - books.google.com
Leading experts from all over the world present an overview of the use of enzymes in
industry for:-the production of bulk products, such as glucose, or fructose-food processing …
industry for:-the production of bulk products, such as glucose, or fructose-food processing …