Directed evolution: methodologies and applications

Y Wang, P Xue, M Cao, T Yu, ST Lane… - Chemical reviews, 2021 - ACS Publications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

A primer to directed evolution: current methodologies and future directions

LS Vidal, M Isalan, JT Heap… - RSC Chemical Biology, 2023 - pubs.rsc.org
Directed evolution is one of the most powerful tools for protein engineering and functions by
harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of …

Methods for the directed evolution of proteins

MS Packer, DR Liu - Nature Reviews Genetics, 2015 - nature.com
Directed evolution has proved to be an effective strategy for improving or altering the activity
of biomolecules for industrial, research and therapeutic applications. The evolution of …

Modified nucleic acid aptamers: development, characterization, and biological applications

D Ji, H Feng, SW Liew, CK Kwok - Trends in Biotechnology, 2023 - cell.com
Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural
interactions. To improve the properties and performance of aptamers, modified nucleotides …

Taxonomy, physiology, and natural products of Actinobacteria

EA Barka, P Vatsa, L Sanchez… - Microbiology and …, 2016 - journals.asm.org
Actinobacteria are Gram-positive bacteria with high G+ C DNA content that constitute one of
the largest bacterial phyla, and they are ubiquitously distributed in both aquatic and …

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

A Currin, N Swainston, PJ Day, DB Kell - Chemical Society Reviews, 2015 - pubs.rsc.org
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes

C Engler, R Gruetzner, R Kandzia, S Marillonnet - PloS one, 2009 - journals.plos.org
We have developed a protocol to assemble in one step and one tube at least nine separate
DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained …

Improving industrial yeast strains: exploiting natural and artificial diversity

J Steensels, T Snoek, E Meersman… - FEMS microbiology …, 2014 - academic.oup.com
Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum

M Ventura, C Canchaya, A Tauch… - Microbiology and …, 2007 - journals.asm.org
Actinobacteria constitute one of the largest phyla among Bacteria and represent gram-
positive bacteria with a high G+ C content in their DNA. This bacterial group includes …

[КНИГА][B] Enzymes in industry: production and applications

W Aehle - 2007 - books.google.com
Leading experts from all over the world present an overview of the use of enzymes in
industry for:-the production of bulk products, such as glucose, or fructose-food processing …