Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

When used appropriately, a confocal fluorescence microscope is an excellent tool for
making quantitative measurements in cells and tissues. The confocal microscope's ability to …

A main determinant of the spatial resolution of live-cell super-resolution (SR) microscopes is
the maximum photon flux that can be collected. To further increase the effective resolution …

Determining the structure and composition of macromolecular assemblies is a major
challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an …

Ribosome biogenesis is among the most resource-intensive cellular processes, with
ribosomal proteins accounting for up to half of all newly synthesized proteins in eukaryotic …

Numerous protocols have been described for producing neural retina from human
pluripotent stem cells (hPSCs), many of which are based on the culture of 3D organoids …

The ability to visualize and quantitatively measure dynamic biological processes in vivo and
at high spatiotemporal resolution is of fundamental importance to experimental …

Experiments in the life sciences often involve tools from a variety of domains such as mass
spectrometry, next generation sequencing, or image processing. Passing the data between …

We establish a murine lung-on-chip infection model and use time-lapse imaging to reveal
the dynamics of host-Mycobacterium tuberculosis interactions at an air-liquid interface with a …

The concomitant occurrence of tissue growth and organization is a hallmark of organismal
development,–. This often means that proliferating and differentiating cells are found at the …