Challenges and opportunities in biological funneling of heterogeneous and toxic substrates beyond lignin

AJ Borchert, WR Henson, GT Beckham - Current opinion in biotechnology, 2022 - Elsevier
Significant developments in the understanding and manipulation of microbial metabolism
have enabled the use of engineered biological systems toward a more sustainable energy …

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes

E Martínez-García, S Fraile, E Algar… - Nucleic Acids …, 2023 - academic.oup.com
Abstract The SEVA platform (https://seva-plasmids. com) was launched one decade ago,
both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …

[HTML][HTML] An update on molecular tools for genetic engineering of actinomycetes—the source of important antibiotics and other valuable compounds

L Mitousis, Y Thoma, EM Musiol-Kroll - Antibiotics, 2020 - mdpi.com
The first antibiotic-producing actinomycete (Streptomyces antibioticus) was described by
Waksman and Woodruff in 1940. This discovery initiated the “actinomycetes era”, in which …

Optimized De Novo Eriodictyol Biosynthesis in Streptomyces albidoflavus Using an Expansion of the Golden Standard Toolkit for Its Use in Actinomycetes

P Magadán-Corpas, S Ye, Á Pérez-Valero… - International Journal of …, 2023 - mdpi.com
Eriodictyol is a hydroxylated flavonoid displaying multiple pharmaceutical activities, such as
antitumoral, antiviral or neuroprotective. However, its industrial production is limited to …

Metabolic engineering in Streptomyces albidoflavus for the biosynthesis of the methylated flavonoids sakuranetin, acacetin, and genkwanin

Á Pérez-Valero, S Ye, P Magadán-Corpas… - Microbial Cell …, 2023 - Springer
Flavonoids are important plant secondary metabolites showing antioxidant, antitumor, anti-
inflammatory, and antiviral activities, among others. Methylated flavonoids are particularly …

Systematic part transfer by extending a modular toolkit to diverse bacteria

KW Keating, EM Young - ACS Synthetic Biology, 2023 - ACS Publications
It is impractical to develop a new parts collection for every potential host organism. It is well-
established that gene expression parts, like genes, are qualitatively transferable, but there is …

Biosynthesis of Hesperetin, Homoeriodictyol, and Homohesperetin in a Transcriptomics-Driven Engineered Strain of Streptomyces albidoflavus

Á Pérez-Valero, J Serna-Diestro… - International Journal of …, 2024 - mdpi.com
Flavonoids exhibit various bioactivities including anti-oxidant, anti-tumor, anti-inflammatory,
and anti-viral properties. Methylated flavonoids are particularly significant due to their …

[HTML][HTML] Systems metabolic engineering of the primary and secondary metabolism of Streptomyces albidoflavus enhances production of the reverse antibiotic …

J Stegmüller, MR Estévez, W Shu, L Gläser… - Metabolic …, 2024 - Elsevier
Nybomycin is an antibiotic compound with proven activity against multi-resistant
Staphylococcus aureus, making it an interesting candidate for combating these globally …

De novo biosynthesis of garbanzol and fustin in Streptomyces albus based on a potential flavanone 3‐hydroxylase with 2‐hydroxylase side activity

L Marín, I Gutiérrez‐del‐Río, CJ Villar… - Microbial …, 2021 - Wiley Online Library
Flavonoids are important plant secondary metabolites, which were shown to have
antioxidant, anti‐inflammatory or antiviral activities. Heterologous production of flavonoids in …

Anti-capsule human monoclonal antibodies protect against hypervirulent and pandrug-resistant Klebsiella pneumoniae

E Roscioli, VZ Galli Fonseca, SS Bosch, I Paciello… - bioRxiv, 2024 - biorxiv.org
The silent pandemic caused by antimicrobial resistance (AMR) requires innovative
therapeutic approaches. Human monoclonal antibodies (mAbs), which are among the most …