Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …

Thanks to the synthetic biology, the laborious and restrictive procedure for producing a
target protein in living microorganisms by biotechnological approaches can now experience …

Engineering regulatory parts for improved performance in genetic programs has played a
pivotal role in the development of the synthetic biology cell programming toolbox. Here, we …

Cell-free expression systems (CFEs) are cutting-edge research tools used in the
investigation of biological phenomena and the engineering of novel biotechnologies. While …

The aim of this study was to investigate if the high substrate turnover rates of Vibrio
natriegens enable 2, 3-butanediol production at high productivities. Introduction of a …

Over the last decades, cell‐free systems have been extensively used for in vitro protein
expression. A vast range of protocols and cellular sources varying from prokaryotes and …

A wide variety of peptidomimetics (peptide analogs) possessing innovative biological
functions have been brought forth as therapeutic candidates through cell-free protein …

Cell-free systems present a significant opportunity to harness the metabolic potential of
diverse organisms. Removing the cellular context provides the ability to produce biological …

Cell-free gene expression (CFE) systems are an attractive tool for engineering within
synthetic biology and for industrial production of high-value recombinant proteins. CFE …

Viruses are the most abundant and diverse biological entities on the planet and constitute a
significant proportion of Earth's genetic diversity. Most of this diversity is not represented by …