Current advances in 3D dynamic cell culture systems

X Huang, Z Huang, W Gao, W Gao, R He, Y Li… - Gels, 2022 - mdpi.com
The traditional two-dimensional (2D) cell culture methods have a long history of mimicking in
vivo cell growth. However, these methods cannot fully represent physiological conditions …

Influence of microenvironment on mesenchymal stem cell therapeutic potency: from planar culture to microcarriers

AC Tsai, R Jeske, X Chen, X Yuan, Y Li - Frontiers in Bioengineering …, 2020 - frontiersin.org
Human mesenchymal stem cells (hMSCs) are a promising candidate in cell therapy as they
exhibit multilineage differentiation, homing to the site of injury, and secretion of trophic …

Maintaining bovine satellite cells stemness through p38 pathway

S Ding, GNM Swennen, T Messmer, M Gagliardi… - Scientific reports, 2018 - nature.com
Isolating and maintaining the appropriate stem cell for large scale cell culture is essential in
tissue engineering or food production. For bovine satellite cells an optimized isolation and …

Attachment and detachment strategies in microcarrier-based cell culture technology: A comprehensive review

S Derakhti, SH Safiabadi-Tali, G Amoabediny… - Materials Science and …, 2019 - Elsevier
Achieving a high cell density of animal cells is a prerequisite for different medical
applications such as cell therapy, tissue engineering, and vaccine production. Microcarrier …

High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling

F Manstein, K Ullmann, C Kropp… - Stem cells …, 2021 - academic.oup.com
To harness the full potential of human pluripotent stem cells (hPSCs) we combined
instrumented stirred tank bioreactor (STBR) technology with the power of in silico process …

Efficient large-scale 2D culture system for human induced pluripotent stem cells and differentiated cardiomyocytes

S Tohyama, J Fujita, C Fujita, M Yamaguchi… - Stem cell reports, 2017 - cell.com
Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are
hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture …

[HTML][HTML] Bioengineering outlook on cultivated meat production

I Pajčin, T Knežić, I Savic Azoulay, V Vlajkov… - Micromachines, 2022 - mdpi.com
Cultured meat (also referred to as cultivated meat or cell-based meat)—CM—is fabricated
through the process of cellular agriculture (CA), which entails application of bioengineering …

Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors

BS Borys, T Dang, T So, L Rohani, T Revay… - Stem Cell Research & …, 2021 - Springer
Background Human induced pluripotent stem cells (hiPSCs) hold enormous promise in
accelerating breakthroughs in understanding human development, drug screening, disease …

Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation

JV Reddy, K Raudenbush, ET Papoutsakis… - Biotechnology …, 2023 - Elsevier
In order to meet the rising demand for biologics and become competitive on the develo**
biosimilar market, there is a need for process intensification of biomanufacturing processes …

Biological considerations in scaling up therapeutic cell manufacturing

DS Cherian, T Bhuvan, L Meagher… - Frontiers in …, 2020 - frontiersin.org
Cell therapeutics—using cells as living drugs—have made advances in many areas of
medicine. One of the most clinically studied cell-based therapy products is mesenchymal …