Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review

J Lü, C Yang, Y Zhang, H Pan - Frontiers in physiology, 2018 - frontiersin.org
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying expression levels of targeted genes during various biological …

Changes in both trans- and cis-regulatory elements mediate insecticide resistance in a lepidopteron pest, Spodoptera exigua

B Hu, H Huang, S Hu, M Ren, Q Wei, X Tian… - PLoS …, 2021 - journals.plos.org
The evolution of insect resistance to insecticides is frequently associated with
overexpression of one or more cytochrome P450 enzyme genes. Although overexpression …

Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted Bollworm Earias vittella (Lepidoptera: Nolidae)

S Sandal, S Singh, G Bansal, R Kaur… - International Journal of …, 2023 - mdpi.com
The spotted bollworm Earias vittella (Lepidoptera: Nolidae) is a polyphagous pest with
enormous economic significance, primarily affecting cotton and okra. However, the lack of …

The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides

B Hu, SH Zhang, MM Ren, XR Tian, Q Wei… - Insect …, 2019 - Wiley Online Library
Cytochrome P450 and UDP‐glucosyltransferase (UGT) as phase I and phase II metabolism
enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics …

Molecular characterization of Cry1F resistance in fall armyworm, Spodoptera frugiperda from Brazil

D Boaventura, J Ulrich, B Lueke, A Bolzan… - Insect biochemistry and …, 2020 - Elsevier
Fall armyworm, Spodoptera frugiperda (JE Smith) is a major lepidopteran pest of maize in
Brazil and its control particularly relies on the use of genetically engineered crops …

Insecticides induce the co-expression of glutathione S-transferases through ROS/CncC pathway in Spodoptera exigua

B Hu, S Hu, H Huang, Q Wei, M Ren, S Huang… - Pesticide Biochemistry …, 2019 - Elsevier
Glutathione S-transferases (GSTs) are a family of multifunctional enzymes that are involved
in detoxification of electrophilic toxic compounds. Although the co-induced expression of …

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in Helicoverpa armigera (Lepidoptera: Noctuidae)

S Zhang, S An, Z Li, F Wu, Q Yang, Y Liu, J Cao… - Gene, 2015 - Elsevier
Background Recent studies have focused on determining functional genes and microRNAs
in the pest Helicoverpa armigera (Lepidoptera: Noctuidae). Most of these studies used …

Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti

N Dzaki, KN Ramli, A Azlan, IH Ishak, G Azzam - Scientific Reports, 2017 - nature.com
Abstract The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-
causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic …

Accelerated delivery of dsRNA in lepidopteran midgut cells by a Galanthus nivalis lectin (GNA)-dsRNA-binding domain fusion protein

Z Martinez, K De Schutter, EJM Van Damme… - Pesticide Biochemistry …, 2021 - Elsevier
Lepidopteran insects are highly refractory to oral RNA interference (RNAi). Degradation,
impaired cellular uptake and intracellular transport of double-stranded RNA (dsRNA) are …

Identification and Validation of Reference Genes for the Normalization of Gene Expression Data in qRT-PCR Analysis in Aphis gossypii (Hemiptera: Aphididae)

KS Ma, F Li, PZ Liang, XW Chen, Y Liu… - Journal of Insect …, 2016 - academic.oup.com
To obtain accurate and reliable results from quantitative real-time reverse transcription
polymerase chain reaction (qRT-PCR) analysis, it is necessary to select suitable reference …