Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

The mammalian retina consists of neurons of> 60 distinct types, each playing a specific role
in processing visual images. They are arranged in three main stages. The first decomposes …

A key challenge in neuroscience is the expeditious reconstruction of neuronal circuits. For
model systems such as Drosophila and C. elegans, the limiting step is no longer the …

Fiji is a distribution of the popular open-source software ImageJ focused on biological-image
analysis. Fiji uses modern software engineering practices to combine powerful software …

Retinitis Pigmentosa (RP) in the human is a progressive, currently irreversible neural
degenerative disease usually caused by gene defects that disrupt the function or …

In the cerebral cortex, local circuits consist of tens of thousands of neurons, each of which
makes thousands of synaptic connections. Perhaps the biggest impediment to …

Groundbreaking advances in volume electron microscopy and specimen preparation are
enabling the 3-dimensional visualisation of specimens with unprecedented detail, and …

The analysis of microcircuitry (the connectivity at the level of individual neuronal processes
and synapses), which is indispensable for our understanding of brain function, is based on …

Anatomy of large biological specimens is often reconstructed from serially sectioned
volumes imaged by high-resolution microscopy. We developed a method to reassemble a …

A lack of methods for measuring the protein compositions of individual synapses in situ has
so far hindered the exploration and exploitation of synapse molecular diversity. Here, we …