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[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …
Molecular photoswitches in aqueous environments
Molecular photoswitches enable dynamic control of processes with high spatiotemporal
precision, using light as external stimulus, and hence are ideal tools for different research …
precision, using light as external stimulus, and hence are ideal tools for different research …
Synthetic biology: bottom-up assembly of molecular systems
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …
opportunities to engineer artificial vesicular systems for applications with previously unmet …
Native chemical ligation and extended methods: mechanisms, catalysis, scope, and limitations
V Agouridas, O El Mahdi, V Diemer, M Cargoet… - Chemical …, 2019 - ACS Publications
The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …
[HTML][HTML] Immobilized enzymes in biosensor applications
Enzyme-based biosensing devices have been extensively developed over the last few
decades, and have proven to be innovative techniques in the qualitative and quantitative …
decades, and have proven to be innovative techniques in the qualitative and quantitative …
Bioconjugation with maleimides: a useful tool for chemical biology
Maleimide chemistry stands out in the bioconjugation toolbox by virtue of its synthetic
accessibility, excellent reactivity, and practicability. The second‐generation of clinically …
accessibility, excellent reactivity, and practicability. The second‐generation of clinically …
Residue-specific peptide modification: a chemist's guide
Advances in bioconjugation and native protein modification are appearing at a blistering
pace, making it increasingly time consuming for practitioners to identify the best chemical …
pace, making it increasingly time consuming for practitioners to identify the best chemical …
Challenges in the use of sortase and other peptide ligases for site-specific protein modification
Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …
many challenges associated with the development of protein modification techniques, in …
Chemistry for covalent modification of endogenous/native proteins: from test tubes to complex biological systems
Chemical modification of proteins provides powerful tools to realize a broad range of
exciting biological applications, including the development of new classes of …
exciting biological applications, including the development of new classes of …
Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …
functions. Understanding the complexities of protein higher-order structures and dynamics …