[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Molecular photoswitches in aqueous environments

J Volarić, W Szymanski, NA Simeth… - Chemical Society …, 2021 - pubs.rsc.org
Molecular photoswitches enable dynamic control of processes with high spatiotemporal
precision, using light as external stimulus, and hence are ideal tools for different research …

Synthetic biology: bottom-up assembly of molecular systems

S Hirschi, TR Ward, WP Meier, DJ Müller… - Chemical …, 2022 - ACS Publications
The bottom-up assembly of biological and chemical components opens exciting
opportunities to engineer artificial vesicular systems for applications with previously unmet …

Native chemical ligation and extended methods: mechanisms, catalysis, scope, and limitations

V Agouridas, O El Mahdi, V Diemer, M Cargoet… - Chemical …, 2019 - ACS Publications
The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

[HTML][HTML] Immobilized enzymes in biosensor applications

HH Nguyen, SH Lee, UJ Lee, CD Fermin, M Kim - Materials, 2019 - mdpi.com
Enzyme-based biosensing devices have been extensively developed over the last few
decades, and have proven to be innovative techniques in the qualitative and quantitative …

Bioconjugation with maleimides: a useful tool for chemical biology

JMJM Ravasco, H Faustino, A Trindade… - … –A European Journal, 2019 - Wiley Online Library
Maleimide chemistry stands out in the bioconjugation toolbox by virtue of its synthetic
accessibility, excellent reactivity, and practicability. The second‐generation of clinically …

Residue-specific peptide modification: a chemist's guide

JN DeGruyter, LR Malins, PS Baran - Biochemistry, 2017 - ACS Publications
Advances in bioconjugation and native protein modification are appearing at a blistering
pace, making it increasingly time consuming for practitioners to identify the best chemical …

Challenges in the use of sortase and other peptide ligases for site-specific protein modification

HE Morgan, WB Turnbull, ME Webb - Chemical Society Reviews, 2022 - pubs.rsc.org
Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Chemistry for covalent modification of endogenous/native proteins: from test tubes to complex biological systems

T Tamura, I Hamachi - Journal of the American Chemical Society, 2018 - ACS Publications
Chemical modification of proteins provides powerful tools to realize a broad range of
exciting biological applications, including the development of new classes of …

Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications

XR Liu, MM Zhang, ML Gross - Chemical reviews, 2020 - ACS Publications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …