Engineering islets from stem cells for advanced therapies of diabetes

J Siehler, AK Blöchinger, M Meier… - Nature reviews Drug …, 2021 - nature.com
Diabetes mellitus is a metabolic disorder that affects more than 460 million people
worldwide. Type 1 diabetes (T1D) is caused by autoimmune destruction of β-cells, whereas …

Towards a better understanding of diabetes mellitus using organoid models

BS Beydag-Tasöz, S Yennek… - Nature Reviews …, 2023 - nature.com
Our understanding of diabetes mellitus has benefited from a combination of clinical
investigations and work in model organisms and cell lines. Organoid models for a wide …

[HTML][HTML] Generation of functional human pancreatic β cells in vitro

FW Pagliuca, JR Millman, M Gürtler, M Segel… - Cell, 2014 - cell.com
The generation of insulin-producing pancreatic β cells from stem cells in vitro would provide
an unprecedented cell source for drug discovery and cell transplantation therapy in …

Functional coupling of human pancreatic islets and liver spheroids on-a-chip: Towards a novel human ex vivo type 2 diabetes model

S Bauer, C Wennberg Huldt, KP Kanebratt, I Durieux… - Scientific reports, 2017 - nature.com
Human in vitro physiological models studying disease and drug treatment effects are
urgently needed as more relevant tools to identify new drug targets and therapies. We have …

[HTML][HTML] Long-term expansion of pancreatic islet organoids from resident Procr+ progenitors

D Wang, J Wang, L Bai, H Pan, H Feng, H Clevers… - Cell, 2020 - cell.com
It has generally proven challenging to produce functional β cells in vitro. Here, we describe a
previously unidentified protein C receptor positive (Procr+) cell population in adult mouse …

Organoid microphysiological system preserves pancreatic islet function within 3D matrix

SN Patel, M Ishahak, D Chaimov, A Velraj… - Science …, 2021 - science.org
Three-dimensional (3D) multicellular organoids recapitulate the native complexities of
human tissue better than traditional cellular monolayers. As organoids are insufficiently …

In vivo–mimicking microfluidic perfusion culture of pancreatic islet spheroids

Y Jun, JS Lee, S Choi, JH Yang, M Sander… - Science …, 2019 - science.org
Native pancreatic islets interact with neighboring cells by establishing three-dimensional
(3D) structures, and are surrounded by perfusion at an interstitial flow level. However, flow …

Recent advances of biosensor-integrated organ-on-a-chip technologies for diagnostics and therapeutics

A Shinde, K Illath, U Kasiviswanathan… - Analytical …, 2023 - ACS Publications
Conventional preclinical evaluation implemented in 2D monolayer cultures falls short with
multiorgan interactions, while animal systems lack the specificity of the human species …

Type 1 Diabetes Mellitus: A Review on Advances and Challenges in Creating Insulin Producing Devices

SM Rodrigues Oliveira, A Rebocho, E Ahmadpour… - Micromachines, 2023 - mdpi.com
Type 1 diabetes mellitus (T1DM) is the most common autoimmune chronic disease in young
patients. It is caused by the destruction of pancreatic endocrine β-cells that produce insulin …

Modelling the endocrine pancreas in health and disease

M Bakhti, A Böttcher, H Lickert - Nature Reviews Endocrinology, 2019 - nature.com
Diabetes mellitus is a multifactorial disease affecting increasing numbers of patients
worldwide. Progression to insulin-dependent diabetes mellitus is characterized by the loss …