Gene expression involves transcription, translation and the turnover of mRNAs and proteins.
The degree to which protein abundances scale with mRNA levels and the implications in …

Numerous biological processes are concurrently and coordinately active in every living cell.
Each of them encompasses synthetic, catalytic and regulatory functions that are, almost …

Thousands of interactions assemble proteins into modules that impart spatial and functional
organization to the cellular proteome. Through affinity-purification mass spectrometry, we …

Cell types are the basic building blocks of multicellular organisms and are extensively
diversified in animals. Despite recent advances in characterizing cell types, classification …

We have used a mass spectrometry-based proteomic approach to compile an atlas of the
thermal stability of 48,000 proteins across 13 species ranging from archaea to humans and …

Adenosine triphosphate (ATP) plays fundamental roles in cellular biochemistry and was
recently discovered to function as a biological hydrotrope. Here, we use mass spectrometry …

Do young and old protein molecules have the same probability to be degraded? We
addressed this question using metabolic pulse-chase labeling and quantitative mass …

Monitoring drug–target interactions with methods such as the cellular thermal-shift assay
(CETSA) is well established for simple cell systems but remains challenging in vivo. Here we …

A progressive loss of protein homeostasis is characteristic of aging and a driver of
neurodegeneration. To investigate this process quantitatively, we characterized proteome …

The complexity of the functional proteome extends considerably beyond the coding genome,
resulting in millions of proteoforms. Investigation of proteoforms and their functional roles is …