[HTML][HTML] Low-dose metformin targets the lysosomal AMPK pathway through PEN2

T Ma, X Tian, B Zhang, M Li, Y Wang, C Yang, J Wu… - Nature, 2022‏ - nature.com
Metformin, the most prescribed antidiabetic medicine, has shown other benefits such as anti-
ageing and anticancer effects,,–. For clinical doses of metformin, AMP-activated protein …

Effects of dielectric barrier discharge (DBD) cold plasma treatment on physicochemical and functional properties of peanut protein

H Ji, S Dong, F Han, Y Li, G Chen, L Li… - Food and Bioprocess …, 2018‏ - Springer
Peanut protein isolate (PPI) solutions were modified by dielectric barrier discharge (DBD)
cold plasma (CP) treatment. Effects of CP treatment on the solubility, emulsion stability, and …

Identification and characterization of asparagine deamidation in the light chain CDR1 of a humanized IgG1 antibody

J Vlasak, MC Bussat, S Wang, E Wagner-Rousset… - Analytical …, 2009‏ - Elsevier
Despite technological advances, detection of deamidation in large proteins remains a
challenge and the use of orthogonal methods is needed for unequivocal assignment. By a …

Separation and analysis of peptides and proteins

CK Larive, SM Lunte, M Zhong, MD Perkins… - Analytical …, 1999‏ - ACS Publications
This review focuses on selected applications of the separation and analysis of peptides and
proteins published during the period of 1997-1998. Specific topic areas covered include …

Temperature stability of proteins: Analysis of irreversible denaturation using isothermal calorimetry

A Schön, BR Clarkson, M Jaime… - … : Structure, Function, and …, 2017‏ - Wiley Online Library
The structural stability of proteins has been traditionally studied under conditions in which
the folding/unfolding reaction is reversible, since thermodynamic parameters can only be …

[HTML][HTML] Lithocholic acid binds TULP3 to activate sirtuins and AMPK to slow down ageing

Q Qu, Y Chen, Y Wang, W Wang, S Long, HY Yang… - Nature, 2024‏ - nature.com
Lithocholic acid (LCA) is accumulated in mammals during calorie restriction and it can
activate AMP-activated protein kinase (AMPK) to slow down ageing. However, the molecular …

Heat‐induced denaturation and aggregation of ovalbumin at neutral pH described by irreversible first‐order kinetics

M Weijers, PA Barneveld, MA Cohen Stuart… - Protein …, 2003‏ - Wiley Online Library
The heat‐induced denaturation kinetics of two different sources of ovalbumin at pH 7 was
studied by chromatography and differential scanning calorimetry. The kinetics was found to …

Influence of calcium ions on the thermal characteristics of α-amylase from thermophilic Anoxybacillus sp. GXS-BL

SM Liao, GE Liang, J Zhu, BO Lu… - Protein and peptide …, 2019‏ - ingentaconnect.com
Background: α-Amylases are starch-degrading enzymes and used widely, the study on
thermostability of α-amylase is a central requirement for its application in life science and …

Thermally induced conformational changes in horseradish peroxidase

DG Pina, AV Shnyrova, F Gavilanes… - European Journal of …, 2001‏ - Wiley Online Library
Detailed differential scanning calorimetry (DSC), steady‐state tryptophan fluorescence and
far‐UV and visible CD studies, together with enzymatic assays, were carried out to monitor …

DSC and electrophoretic studies on soymilk protein denaturation

H Zhang, M Takenaka, S Isobe - Journal of thermal analysis and …, 2004‏ - Springer
The effects of heat treatment on soymilk protein denaturation were studied by differential
scanning calorimetry (DSC) and electrophoresis. Transition behavior of soymilk was studied …