Large-scale de novo DNA synthesis: technologies and applications
For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …
understand and engineer biology. Here we summarize methods and caveats for the de novo …
Concerning RNA-guided gene drives for the alteration of wild populations
Gene drives may be capable of addressing ecological problems by altering entire
populations of wild organisms, but their use has remained largely theoretical due to …
populations of wild organisms, but their use has remained largely theoretical due to …
Codon bias as a means to fine-tune gene expression
The redundancy of the genetic code implies that most amino acids are encoded by multiple
synonymous codons. In all domains of life, a biased frequency of synonymous codons is …
synonymous codons. In all domains of life, a biased frequency of synonymous codons is …
Design of a synthetic yeast genome
We describe complete design of a synthetic eukaryotic genome, Sc2. 0, a highly modified
Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of …
Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of …
Aminoacyl-tRNA synthetases
MAR Gomez, M Ibba - Rna, 2020 - rnajournal.cshlp.org
The aminoacyl-tRNA synthetases are an essential and universally distributed family of
enzymes that plays a critical role in protein synthesis, pairing tRNAs with their cognate …
enzymes that plays a critical role in protein synthesis, pairing tRNAs with their cognate …
Genomically recoded organisms expand biological functions
We describe the construction and characterization of a genomically recoded organism
(GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with …
(GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with …
Hydrogel-based biocontainment of bacteria for continuous sensing and computation
Genetically modified microorganisms (GMMs) can enable a wide range of important
applications including environmental sensing and responsive engineered living materials …
applications including environmental sensing and responsive engineered living materials …
Cracking the code: reprogramming the genetic script in prokaryotes and eukaryotes to harness the power of noncanonical amino acids
C Jann, S Giofré, R Bhattacharjee, EA Lemke - Chemical Reviews, 2024 - ACS Publications
Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …
decades. Incorporating these noncanonical amino acids into proteins enables many …
Designing logical codon reassignment–Expanding the chemistry in biology
Over the last decade, the ability to genetically encode unnatural amino acids (UAAs) has
evolved rapidly. The programmed incorporation of UAAs into recombinant proteins relies on …
evolved rapidly. The programmed incorporation of UAAs into recombinant proteins relies on …
Design, synthesis, and testing toward a 57-codon genome
Recoding—the repurposing of genetic codons—is a powerful strategy for enhancing
genomes with functions not commonly found in nature. Here, we report computational …
genomes with functions not commonly found in nature. Here, we report computational …