The CRISPR–Cas toolbox for analytical and diagnostic assay development

Y Tang, L Gao, W Feng, C Guo, Q Yang, F Li… - Chemical Society …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …

Signal amplification and output of CRISPR/Cas-based biosensing systems: A review

SY Wang, YC Du, DX Wang, JY Ma, AN Tang… - Analytica Chimica …, 2021 - Elsevier
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) proteins are powerful gene-editing tools because of their ability to accurately …

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

Z Chen, J Li, T Li, T Fan, C Meng, C Li… - National Science …, 2022 - academic.oup.com
The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying
asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong …

Size-controlled engineering photoelectrochemical biosensor for human papillomavirus-16 based on CRISPR-Cas12a-induced disassembly of Z-scheme …

Y Li, R Zeng, W Wang, J Xu, H Gong, L Li, M Li… - ACS …, 2022 - ACS Publications
Photoelectrochemical (PEC) biosensors incorporating biomolecular recognition with photon-
to-electron conversion capabilities of the photoactive species have been developed for …

A highly sensitive CRISPR‐empowered surface plasmon resonance sensor for diagnosis of inherited diseases with femtomolar‐level real‐time quantification

F Zheng, Z Chen, J Li, R Wu, B Zhang, G Nie… - Advanced …, 2022 - Wiley Online Library
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system
has emerged as a promising technology for the detection of nucleic acids. Herein, the …

Photoelectrochemical bioanalysis of microRNA on yolk-in-shell Au@ CdS based on the catalytic hairpin assembly-mediated CRISPR-Cas12a system

R Zeng, J Xu, L Lu, Q Lin, X Huang, L Huang… - Chemical …, 2022 - pubs.rsc.org
This work reports on the proof-of-concept of a photoelectrochemical (PEC) biosensor with a
horseradish peroxidase-single stranded DNA-encoded magnetic bead (MB-ssDNA-HRP) …

CRISPR-Cas12a-empowered electrochemical biosensor for rapid and ultrasensitive detection of SARS-CoV-2 delta variant

C Wu, Z Chen, C Li, Y Hao, Y Tang, Y Yuan, L Chai… - Nano-Micro Letters, 2022 - Springer
Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The gold standard method …

CRISPR/Cas12a-based photoelectrochemical sensing of microRNA on reduced graphene oxide-anchored Bi2WO6 coupling with catalytic hairpin assembly

H Gong, X Hu, R Zeng, Y Li, J Xu, M Li… - Sensors and Actuators B …, 2022 - Elsevier
An innovative and generic CRISPR/Cas12a-driven photoelectrochemical (PEC) biosensing
platform was developed for screening of microRNA-21 (miR-21) by coupling with target …

Cascade amplification strategy based on ultra-thin graphdiyne and CRISPR/Cas for real-time detection of tumor biomarker

J Xu, Y Liu, KJ Huang, R Wang, J Li - Chemical Engineering Journal, 2023 - Elsevier
As an important cancer markers, the sensitive, portable and highly specific detection of
miRNA-141 is impendency. Currently, self-powered electrochemical sensors based on …

Universal and programmable rolling circle amplification-CRISPR/Cas12a-mediated immobilization-free electrochemical biosensor

M Qing, SL Chen, Z Sun, Y Fan, HQ Luo… - Analytical …, 2021 - ACS Publications
The development of a sensing platform with high sensitivity and specificity, especially
programmability and universal applicability, for the detection of clinically relevant molecules …