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Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
CRISPR-Cas13a-powered electrochemical biosensor for the detection of the L452R mutation in clinical samples of SARS-CoV-2 variants
Since the end of 2019, a highly contagious disease caused by severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called …
syndrome coronavirus 2 (SARS-CoV-2) has deprived numerous lives worldwide, called …
CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities
Food safety is one of the biggest public issues occurring around the world. Microbiological,
chemical, and physical hazards can lead to food safety issues, which may occur at all stages …
chemical, and physical hazards can lead to food safety issues, which may occur at all stages …
CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives
Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …
repositioned in nucleic acid detection. A preamplification step is usually required to improve …
Isothermal nucleic acid amplification for food safety analysis
Rapid assays allowing for on-site or in-field testing can enable us to detect food
contamination in a timely manner, thus facilitating the insurance of food safety. Isothermal …
contamination in a timely manner, thus facilitating the insurance of food safety. Isothermal …
State of the art in CRISPR/Cas system-based signal conversion and amplification applied in the field of food analysis
Background Food safety is a major global public health issue. The majority of traditional
culture-based methods have lengthy detection times, require sophisticated sample …
culture-based methods have lengthy detection times, require sophisticated sample …
Signal amplification and output of CRISPR/Cas-based biosensing systems: A review
CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-
associated) proteins are powerful gene-editing tools because of their ability to accurately …
associated) proteins are powerful gene-editing tools because of their ability to accurately …
CRISPR/Cas-engineered technology: Innovative approach for biosensor development
On-site and real-time clinical monitoring have been progressed dramatically by integrating
biosensor science with portable digital electronic technology. Clustered regularly …
biosensor science with portable digital electronic technology. Clustered regularly …
CRISPR-Cas13a system: A novel tool for molecular diagnostics
L Zhao, M Qiu, X Li, J Yang, J Li - Frontiers in Microbiology, 2022 - frontiersin.org
The clustered regularly interspaced short palindromic repeats (CRISPR) system is a natural
adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into …
adaptive immune system of prokaryotes. The CRISPR-Cas system is currently divided into …