Quantitative phase imaging based on holography: trends and new perspectives

Z Huang, L Cao - Light: Science & Applications, 2024 - nature.com
Abstract In 1948, Dennis Gabor proposed the concept of holography, providing a pioneering
solution to a quantitative description of the optical wavefront. After 75 years of development …

Label-free biomedical optical imaging

NT Shaked, SA Boppart, LV Wang, J Popp - Nature photonics, 2023 - nature.com
Label-free optical imaging employs natural and non-destructive approaches to visualize
biomedical samples for both biological assays and clinical diagnosis. At present, this field …

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

D Pirone, J Lim, F Merola, L Miccio, M Mugnano… - Nature …, 2022 - nature.com
Quantitative phase imaging has gained popularity in bioimaging because it can avoid the
need for cell staining, which, in some cases, is difficult or impossible. However, as a result …

Holotomography

G Kim, H Hugonnet, K Kim, JH Lee, SS Lee… - Nature Reviews …, 2024 - nature.com
Holotomography (HT) represents a 3D, label-free optical imaging methodology that
leverages refractive index as an inherent quantitative contrast for imaging. This technique …

Machine learning for sperm selection

JB You, C McCallum, Y Wang, J Riordon… - Nature Reviews …, 2021 - nature.com
Infertility rates and the number of couples seeking fertility care have increased worldwide
over the past few decades. Over 2.5 million cycles of assisted reproductive technologies are …

High spatial and temporal resolution synthetic aperture phase microscopy

C Zheng, D **, Y He, H Lin, J Hu… - Advanced …, 2020 - spiedigitallibrary.org
A new optical microscopy technique, termed high spatial and temporal resolution synthetic
aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of …

Simulating nature in sperm selection for assisted reproduction

ETY Leung, CL Lee, X Tian, KKW Lam, RHW Li… - Nature Reviews …, 2022 - nature.com
Sperm selection in the female reproductive tract (FRT) is sophisticated. Only about 1,000
sperm out of millions in an ejaculate reach the fallopian tube and thus have a chance of …

Multiscale label-free volumetric holographic histopathology of thick-tissue slides with subcellular resolution

H Hugonnet, YW Kim, M Lee, S Shin… - Advanced …, 2021 - spiedigitallibrary.org
Histopathology relies upon the staining and sectioning of biological tissues, which can be
laborious and may cause artifacts and distort tissues. We develop label-free volumetric …

Dehydration of plant cells shoves nuclei rotation allowing for 3D phase-contrast tomography

Z Wang, V Bianco, D Pirone, P Memmolo… - Light: Science & …, 2021 - nature.com
Single-cell phase-contrast tomography promises to become decisive for studying 3D
intracellular structures in biology. It involves probing cells with light at wide angles, which …

Full-field optical coherence microscopy enables high-resolution label-free imaging of the dynamics of live mouse oocytes and early embryos

S Morawiec, A Ajduk, P Stremplewski… - Communications …, 2024 - nature.com
High quality label-free imaging of oocytes and early embryos is essential for accurate
assessment of their developmental potential, a key element of assisted reproduction …