The field of protein design has made remarkable progress over the past decade. Historically,
the low reliability of purely structure-based design methods limited their application, but …

The prediction of protein three-dimensional structure from amino acid sequence has been a
grand challenge problem in computational biophysics for decades, owing to its intrinsic …

Nature has provided a fantastic array of enzymes that are responsible for essential
biochemical functions but not usually suitable for technological applications. Not content …

Amino acid mutations that lower a protein's thermodynamic stability are implicated in
numerous diseases, and engineered proteins with enhanced stability can be important in …

The term B-factor, sometimes called the Debye–Waller factor, temperature factor, or atomic
displacement parameter, is used in protein crystallography to describe the attenuation of X …

Designing efficient enzymes is a formidable challenge at the forefront of modern
biocatalysis. Here, we review recent developments in the field and illustrate how the …

Thermostable proteins find their use in numerous biomedical and biotechnological
applications. However, the computational design of stable proteins often results in single …

HotSpot Wizard is a web server used for the automated identification of hotspots in semi-
rational protein design to give improved protein stability, catalytic activity, substrate …

Thermostability is considered to be an important parameter to measure the feasibility of
enzymes for industrial applications. Generally, higher thermostability makes an enzyme …

Improving protein stability is an important goal for basic research as well as for clinical and
industrial applications but no commonly accepted and widely used strategy for efficient …