Isolation and culture of adult neurons and neurospheres

GJ Brewer, JR Torricelli - Nature protocols, 2007 - nature.com
Here we present a protocol for extraction and culture of neurons from adult rat or mouse
CNS. The method proscribes an optimized protease digestion of slices, control of osmolarity …

High efficacy of clonal growth and expansion of adult neural stem cells

FP Wachs, S Couillard-Despres, M Engelhardt… - Laboratory …, 2003 - nature.com
Neural stem cells (NSCs) from the adult central nervous system are currently being
investigated for their potential use in autologous cell replacement strategies. High …

Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique

I Manjubala, S Scheler, J Bössert, KD Jandt - Acta biomaterialia, 2006 - Elsevier
The study of inorganic crystal assembly in organic matrices has given rise to increasing
interest in various fields of materials science to the natural process of biomineralisation. To …

Long-term proliferation of human embryonic stem cell–derived neuroepithelial cells using defined adherent culture conditions

S Shin, M Mitalipova, S Noggle, D Tibbitts… - Stem …, 2006 - academic.oup.com
Research on the cell fate determination of embryonic stem cells is of enormous interest
given the therapeutic potential in regenerative cell therapy. Human embryonic stem cells …

Mouse retina explants after long-term culture in serum free medium

AR Caffe, P Ahuja, B Holmqvist, S Azadi… - Journal of chemical …, 2002 - Elsevier
The neonatal mouse retina remains viable as an explant in serum-supplemented growth
media for more than 4 weeks. Interpretation of drug effects on this tissue is compromised by …

Isolation and culture of rat embryonic neural cells: a quick protocol

M Pacifici, F Peruzzi - Journal of visualized experiments: JoVE, 2012 - pmc.ncbi.nlm.nih.gov
We are describing a quick method to dissociate and culture hippocampal or cortical neurons
from E15-17 rat embryos. The procedure can be applied successfully to the isolation of …

Mitochondrial hexokinase II promotes neuronal survival and acts downstream of glycogen synthase kinase-3

A Gimenez-Cassina, F Lim, T Cerrato… - Journal of Biological …, 2009 - jbc.org
Mitochondrial alterations are detected in most neurodegenerative disorders and may
contribute to the dysfunction and demise of neuronal cells. Because glycogen synthase …

Bisphenol A promotes dendritic morphogenesis of hippocampal neurons through estrogen receptor-mediated ERK1/2 signal pathway

X Xu, Y Lu, G Zhang, L Chen, D Tian, X Shen, Y Yang… - Chemosphere, 2014 - Elsevier
Bisphenol A (BPA), an environmental endocrine disruptor, has attracted increasing attention
to its adverse effects on brain developmental process. The previous study indicated that BPA …

The effect of hypothermic and cryogenic preservation on engineered neural tissue

AGE Day, KS Bhangra, C Murray-Dunning… - … Engineering Part C …, 2017 - liebertpub.com
This study explored different approaches to preserve engineered neural tissue (EngNT), a
stabilized, cellular collagen hydrogel containing columns of aligned Schwann cells for …

Bisphenol-A rapidly promotes dynamic changes in hippocampal dendritic morphology through estrogen receptor-mediated pathway by concomitant phosphorylation …

X Xu, Y Ye, T Li, L Chen, D Tian, Q Luo, M Lu - Toxicology and applied …, 2010 - Elsevier
Bisphenol-A (BPA) is known to be a potent endocrine disrupter. Evidence is emerging that
estrogen exerts a rapid influence on hippocampal synaptic plasticity and the dendritic spine …