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Isolation and culture of adult neurons and neurospheres
GJ Brewer, JR Torricelli - Nature protocols, 2007 - nature.com
Here we present a protocol for extraction and culture of neurons from adult rat or mouse
CNS. The method proscribes an optimized protease digestion of slices, control of osmolarity …
CNS. The method proscribes an optimized protease digestion of slices, control of osmolarity …
High efficacy of clonal growth and expansion of adult neural stem cells
Neural stem cells (NSCs) from the adult central nervous system are currently being
investigated for their potential use in autologous cell replacement strategies. High …
investigated for their potential use in autologous cell replacement strategies. High …
Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique
I Manjubala, S Scheler, J Bössert, KD Jandt - Acta biomaterialia, 2006 - Elsevier
The study of inorganic crystal assembly in organic matrices has given rise to increasing
interest in various fields of materials science to the natural process of biomineralisation. To …
interest in various fields of materials science to the natural process of biomineralisation. To …
Long-term proliferation of human embryonic stem cell–derived neuroepithelial cells using defined adherent culture conditions
S Shin, M Mitalipova, S Noggle, D Tibbitts… - Stem …, 2006 - academic.oup.com
Research on the cell fate determination of embryonic stem cells is of enormous interest
given the therapeutic potential in regenerative cell therapy. Human embryonic stem cells …
given the therapeutic potential in regenerative cell therapy. Human embryonic stem cells …
Mouse retina explants after long-term culture in serum free medium
AR Caffe, P Ahuja, B Holmqvist, S Azadi… - Journal of chemical …, 2002 - Elsevier
The neonatal mouse retina remains viable as an explant in serum-supplemented growth
media for more than 4 weeks. Interpretation of drug effects on this tissue is compromised by …
media for more than 4 weeks. Interpretation of drug effects on this tissue is compromised by …
Isolation and culture of rat embryonic neural cells: a quick protocol
M Pacifici, F Peruzzi - Journal of visualized experiments: JoVE, 2012 - pmc.ncbi.nlm.nih.gov
We are describing a quick method to dissociate and culture hippocampal or cortical neurons
from E15-17 rat embryos. The procedure can be applied successfully to the isolation of …
from E15-17 rat embryos. The procedure can be applied successfully to the isolation of …
Mitochondrial hexokinase II promotes neuronal survival and acts downstream of glycogen synthase kinase-3
A Gimenez-Cassina, F Lim, T Cerrato… - Journal of Biological …, 2009 - jbc.org
Mitochondrial alterations are detected in most neurodegenerative disorders and may
contribute to the dysfunction and demise of neuronal cells. Because glycogen synthase …
contribute to the dysfunction and demise of neuronal cells. Because glycogen synthase …
Bisphenol A promotes dendritic morphogenesis of hippocampal neurons through estrogen receptor-mediated ERK1/2 signal pathway
X Xu, Y Lu, G Zhang, L Chen, D Tian, X Shen, Y Yang… - Chemosphere, 2014 - Elsevier
Bisphenol A (BPA), an environmental endocrine disruptor, has attracted increasing attention
to its adverse effects on brain developmental process. The previous study indicated that BPA …
to its adverse effects on brain developmental process. The previous study indicated that BPA …
The effect of hypothermic and cryogenic preservation on engineered neural tissue
AGE Day, KS Bhangra, C Murray-Dunning… - … Engineering Part C …, 2017 - liebertpub.com
This study explored different approaches to preserve engineered neural tissue (EngNT), a
stabilized, cellular collagen hydrogel containing columns of aligned Schwann cells for …
stabilized, cellular collagen hydrogel containing columns of aligned Schwann cells for …
Bisphenol-A rapidly promotes dynamic changes in hippocampal dendritic morphology through estrogen receptor-mediated pathway by concomitant phosphorylation …
X Xu, Y Ye, T Li, L Chen, D Tian, Q Luo, M Lu - Toxicology and applied …, 2010 - Elsevier
Bisphenol-A (BPA) is known to be a potent endocrine disrupter. Evidence is emerging that
estrogen exerts a rapid influence on hippocampal synaptic plasticity and the dendritic spine …
estrogen exerts a rapid influence on hippocampal synaptic plasticity and the dendritic spine …