The Mediator complex as a master regulator of transcription by RNA polymerase II

WF Richter, S Nayak, J Iwasa, DJ Taatjes - Nature Reviews Molecular …, 2022 - nature.com
The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

[HTML][HTML] The self-organizing genome: principles of genome architecture and function

T Misteli - Cell, 2020 - cell.com
Genomes have complex three-dimensional architectures. The recent convergence of
genetic, biochemical, biophysical, and cell biological methods has uncovered several …

Mechanisms of enhancer action: the known and the unknown

A Panigrahi, BW O'Malley - Genome biology, 2021 - Springer
Differential gene expression mechanisms ensure cellular differentiation and plasticity to
shape ontogenetic and phylogenetic diversity of cell types. A key regulator of differential …

[HTML][HTML] “Structure”-function relationships in eukaryotic transcription factors: the role of intrinsically disordered regions in gene regulation

JJ Ferrie, JP Karr, R Tjian, X Darzacq - Molecular Cell, 2022 - cell.com
Many principles of bacterial gene regulation have been foundational to understanding
mechanisms of eukaryotic transcription. However, stark structural and functional differences …

[HTML][HTML] Structure and mechanism of the RNA polymerase II transcription machinery

AC Schier, DJ Taatjes - Genes & development, 2020 - genesdev.cshlp.org
Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …

Illuminating RNA biology through imaging

P Le, N Ahmed, GW Yeo - Nature cell biology, 2022 - nature.com
RNA processing plays a central role in accurately transmitting genetic information into
functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to …

Transcription in living cells: molecular mechanisms of bursting

J Rodriguez, DR Larson - Annual review of biochemistry, 2020 - annualreviews.org
Transcription in several organisms from certain bacteria to humans has been observed to be
stochastic in nature: toggling between active and inactive states. Periods of active nascent …

What is a transcriptional burst?

E Tunnacliffe, JR Chubb - Trends in Genetics, 2020 - cell.com
The idea that gene activity can be discontinuous will not surprise many biologists–many
genes are restricted in when and where they can be expressed. Yet during the past 15 …

Transcription factor dynamics: one molecule at a time

K Wagh, DA Stavreva, A Upadhyaya… - Annual Review of Cell …, 2023 - annualreviews.org
Cells must tightly regulate their gene expression programs and yet rapidly respond to acute
biochemical and biophysical cues within their environment. This information is transmitted to …

An intrinsically disordered region-mediated confinement state contributes to the dynamics and function of transcription factors

DA Garcia, TA Johnson, DM Presman, G Fettweis… - Molecular cell, 2021 - cell.com
Transcription factors (TFs) regulate gene expression by binding to specific consensus motifs
within the local chromatin context. The mechanisms by which TFs navigate the nuclear …