Coacervate droplets for synthetic cells

Z Lin, T Beneyton, JC Baret, N Martin - Small Methods, 2023 - Wiley Online Library
The design and construction of synthetic cells–human‐made microcompartments that mimic
features of living cells–have experienced a real boom in the past decade. While many efforts …

Growth, replication and division enable evolution of coacervate protocells

AD Slootbeek, MHI van Haren, IBA Smokers… - Chemical …, 2022 - pubs.rsc.org
Living and proliferating cells undergo repeated cycles of growth, replication and division, all
orchestrated by complex molecular networks. How a minimal cell cycle emerged and helped …

Endocytosis of coacervates into liposomes

T Lu, S Liese, L Schoenmakers, CA Weber… - Journal of the …, 2022 - ACS Publications
Recent studies have shown that the interactions between condensates and biological
membranes are of functional importance. Here, we study how the interaction between …

Plant cell-inspired membranization of coacervate protocells with a structured polysaccharide layer

Y Ji, Y Lin, Y Qiao - Journal of the American Chemical Society, 2023 - ACS Publications
The design of compartmentalized colloids that exhibit biomimetic properties is providing
model systems for develo** synthetic cell-like entities (protocells). Inspired by the cell …

Triggerable protocell capture in nanoparticle-caged coacervate microdroplets

N Gao, C Xu, Z Yin, M Li, S Mann - Journal of the American …, 2022 - ACS Publications
Controlling the dynamics of mixed communities of cell-like entities (protocells) provides a
step toward the development of higher-order cytomimetic behaviors in artificial cell …

[HTML][HTML] Can coacervation unify disparate hypotheses in the origin of cellular life?

B Ghosh, R Bose, TYD Tang - Current opinion in colloid & interface science, 2021 - Elsevier
Here, we review the recent progress in the characterisation and utilisation of coacervates as
protocell models in origin of life studies. We provide evidence that coacervation could have …

Prebiotically-relevant low polyion multivalency can improve functionality of membraneless compartments

FP Cakmak, S Choi, MCO Meyer… - Nature …, 2020 - nature.com
Multivalent polyions can undergo complex coacervation, producing membraneless
compartments that accumulate ribozymes and enhance catalysis, and offering a mechanism …

Microscopic imaging techniques for molecular assemblies: electron, atomic force, and confocal microscopies

R Kubota, W Tanaka, I Hamachi - Chemical Reviews, 2021 - ACS Publications
Self-assembly is promising for construction of a wide variety of supramolecular assemblies,
whose 1D/2D/3D structures are typically relevant to their functions. In-depth understanding …

Building micro-capsules using water-in-water emulsion droplets as templates

A Perro, N Coudon, JP Chapel, N Martin… - Journal of Colloid and …, 2022 - Elsevier
The use of templates in materials chemistry is a well-established approach for producing
membrane-bounded hollow spheres used for microencapsulation applications, but also in …

Biomolecular condensates in contact with membranes

A Mangiarotti, R Dimova - Annual Review of Biophysics, 2024 - annualreviews.org
Biomolecular condensates are highly versatile membraneless organelles involved in a
plethora of cellular processes. Recent years have witnessed growing evidence of the …