Applications of deep learning in understanding gene regulation

Z Li, E Gao, J Zhou, W Han, X Xu, X Gao - Cell Reports Methods, 2023 - cell.com
Gene regulation is a central topic in cell biology. Advances in omics technologies and the
accumulation of omics data have provided better opportunities for gene regulation studies …

Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation

J Linder, D Srivastava, H Yuan, V Agarwal, DR Kelley - Nature Genetics, 2025 - nature.com
Sequence-based machine-learning models trained on genomics data improve genetic
variant interpretation by providing functional predictions describing their impact on the cis …

The role of alternative polyadenylation in the regulation of subcellular RNA localization

A Arora, R Goering, HYG Lo, J Lo, C Moffatt… - Frontiers in …, 2022 - frontiersin.org
Alternative polyadenylation (APA) is a widespread and conserved regulatory mechanism
that generates diverse 3′ ends on mRNA. APA patterns are often tissue specific and play …

Context-aware poly (a) signal prediction model via deep spatial–temporal neural networks

Y Guo, D Zhou, P Li, C Li, J Cao - IEEE Transactions on Neural …, 2022 - ieeexplore.ieee.org
Polyadenylation [Poly (A)] is an essential process during messenger RNA (mRNA)
maturation in biological eukaryote systems. Identifying Poly (A) signals (PASs) from the …

Multiplexed single-cell characterization of alternative polyadenylation regulators

MH Kowalski, HH Wessels, J Linder, C Dalgarno… - Cell, 2024 - cell.com
Most mammalian genes have multiple polyA sites, representing a substantial source of
transcript diversity regulated by the cleavage and polyadenylation (CPA) machinery. To …

Deciphering the impact of genetic variation on human polyadenylation using APARENT2

J Linder, SE Koplik, A Kundaje, G Seelig - Genome biology, 2022 - Springer
Background 3′-end processing by cleavage and polyadenylation is an important and finely
tuned regulatory process during mRNA maturation. Numerous genetic variants are known to …

Fast activation maximization for molecular sequence design

J Linder, G Seelig - BMC bioinformatics, 2021 - Springer
Background Optimization of DNA and protein sequences based on Machine Learning
models is becoming a powerful tool for molecular design. Activation maximization offers a …

A survey on methods for predicting polyadenylation sites from DNA sequences, bulk RNA-seq, and single-cell RNA-seq

W Ye, Q Lian, C Ye, X Wu - Genomics, Proteomics & …, 2023 - academic.oup.com
Alternative polyadenylation (APA) plays important roles in modulating mRNA stability,
translation, and subcellular localization, and contributes extensively to sha** eukaryotic …

DeepGenGrep: a general deep learning-based predictor for multiple genomic signals and regions

Q Liu, H Fang, X Wang, M Wang, S Li, LJM Coin… - …, 2022 - academic.oup.com
Motivation Accurate annotation of different genomic signals and regions (GSRs) from DNA
sequences is fundamentally important for understanding gene structure, regulation and …

Interpreting neural networks for biological sequences by learning stochastic masks

J Linder, A La Fleur, Z Chen, A Ljubetič… - Nature machine …, 2022 - nature.com
Sequence-based neural networks can learn to make accurate predictions from large
biological datasets, but model interpretation remains challenging. Many existing feature …