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Limitations and opportunities of technologies for the analysis of cell-free DNA in cancer diagnostics
Cell-free DNA (cfDNA) in the circulating blood plasma of patients with cancer contains
tumour-derived DNA sequences that can serve as biomarkers for guiding therapy, for the …
tumour-derived DNA sequences that can serve as biomarkers for guiding therapy, for the …
dPCR: a technology review
Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …
The environmental microbiology minimum information (EMMI) guidelines: qPCR and dPCR quality and reporting for environmental microbiology
Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods
have revolutionized environmental microbiology, yielding quantitative organism-specific …
have revolutionized environmental microbiology, yielding quantitative organism-specific …
Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold
standard method for the diagnosis of COVID-19 infection. Due to pre-analytical and …
standard method for the diagnosis of COVID-19 infection. Due to pre-analytical and …
The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020
JF Huggett - Clinical chemistry, 2020 - academic.oup.com
Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …
Microfluidics-based strategies for molecular diagnostics of infectious diseases
Traditional diagnostic strategies for infectious disease detection require benchtop
instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a …
instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a …
Digital assays part I: partitioning statistics and digital PCR
AS Basu - SLAS TECHNOLOGY: Translating Life Sciences …, 2017 - journals.sagepub.com
A digital assay is one in which the sample is partitioned into many small containers such that
each partition contains a discrete number of biological entities (0, 1, 2, 3,…). A powerful …
each partition contains a discrete number of biological entities (0, 1, 2, 3,…). A powerful …
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …
practical and increasingly affordable technology. dPCR allows the precise quantification of …
Emerging digital PCR technology in precision medicine
L Zhang, R Parvin, Q Fan, F Ye - Biosensors and Bioelectronics, 2022 - Elsevier
Digital PCR (dPCR) is built on partitioning reagent to the extent that single template
molecules are amplified and visualized individually, whereby offers higher precision and …
molecules are amplified and visualized individually, whereby offers higher precision and …
Considerations for digital PCR as an accurate molecular diagnostic tool
JF Huggett, S Cowen, CA Foy - Clinical chemistry, 2015 - academic.oup.com
Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …
PCR that offers a number of unique advantages when applied to preclinical research …