Limitations and opportunities of technologies for the analysis of cell-free DNA in cancer diagnostics

P Song, LR Wu, YH Yan, JX Zhang, T Chu… - Nature biomedical …, 2022 - nature.com
Cell-free DNA (cfDNA) in the circulating blood plasma of patients with cancer contains
tumour-derived DNA sequences that can serve as biomarkers for guiding therapy, for the …

dPCR: a technology review

PL Quan, M Sauzade, E Brouzes - Sensors, 2018 - mdpi.com
Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

The environmental microbiology minimum information (EMMI) guidelines: qPCR and dPCR quality and reporting for environmental microbiology

MA Borchardt, AB Boehm, M Salit… - Environmental …, 2021 - ACS Publications
Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods
have revolutionized environmental microbiology, yielding quantitative organism-specific …

Sensitivity assessment of droplet digital PCR for SARS-CoV-2 detection

L Falzone, N Musso, G Gattuso… - International …, 2020 - spandidos-publications.com
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold
standard method for the diagnosis of COVID-19 infection. Due to pre-analytical and …

The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020

JF Huggett - Clinical chemistry, 2020 - academic.oup.com
Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …

Microfluidics-based strategies for molecular diagnostics of infectious diseases

X Wang, XZ Hong, YW Li, Y Li, J Wang, P Chen… - Military Medical …, 2022 - Springer
Traditional diagnostic strategies for infectious disease detection require benchtop
instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics, a …

Digital assays part I: partitioning statistics and digital PCR

AS Basu - SLAS TECHNOLOGY: Translating Life Sciences …, 2017 - journals.sagepub.com
A digital assay is one in which the sample is partitioned into many small containers such that
each partition contains a discrete number of biological entities (0, 1, 2, 3,…). A powerful …

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

JF Huggett, CA Foy, V Benes, K Emslie… - Clinical …, 2013 - academic.oup.com
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …

Emerging digital PCR technology in precision medicine

L Zhang, R Parvin, Q Fan, F Ye - Biosensors and Bioelectronics, 2022 - Elsevier
Digital PCR (dPCR) is built on partitioning reagent to the extent that single template
molecules are amplified and visualized individually, whereby offers higher precision and …

Considerations for digital PCR as an accurate molecular diagnostic tool

JF Huggett, S Cowen, CA Foy - Clinical chemistry, 2015 - academic.oup.com
Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …