DNA topoisomerases are enzymes that disentangle the topological problems that arise in
double-stranded DNA. Many of these can be solved by the generation of either single or …

In recent years, quantification of absolute protein numbers in cellular structures using
fluorescence microscopy has become a reality. Two popular methods are available to a …

Current cancer chemotherapies are limited by the lack of tumor-specific targets, which would
allow for selective eradication of malignant cells without affecting healthy tissues. In contrast …

Accurate segregation of the replicated genome requires chromosome biorientation on the
spindle. Biorientation is ensured by Aurora B kinase (Ipl1), a member of the four-subunit …

Eukaryotic cells segregate their chromosomes accurately to opposite poles during mitosis,
which is necessary for maintenance of their genetic integrity. This process mainly relies on …

The γ-tubulin ring complex (γTuRC) is the primary microtubule nucleator in cells. γγTuRC is
assembled from repeating γγ-tubulin small complex (γTuSC) subunits and is thought to …

The stoichiometries of kinetochores and their constituent proteins in yeast and vertebrate
cells were determined using the histone H3 variant CENP-A, known as Cse4 in budding …

For proper chromosome segregation in mitosis, sister kinetochores must interact with
microtubules from opposite spindle poles (chromosome bi-orientation)[1, 2]. To promote bi …

Both an increased frequency of chromosome missegregation (chromosomal instability, CIN)
and the presence of an abnormal complement of chromosomes (aneuploidy) are hallmarks …

Accurate chromosome segregation depends on sister kinetochores making bioriented
attachments to microtubules from opposite poles. An essential regulator of biorientation is …