RNases H: Structure and mechanism
M Hyjek, M Figiel, M Nowotny - DNA repair, 2019 - Elsevier
RNases H are a family of endonucleases that hydrolyze RNA residues in various nucleic
acids. These enzymes are present in all branches of life, and their counterpart domains are …
acids. These enzymes are present in all branches of life, and their counterpart domains are …
Argonaute2 is the catalytic engine of mammalian RNAi
J Liu, MA Carmell, FV Rivas, CG Marsden… - Science, 2004 - science.org
Gene silencing through RNA interference (RNAi) is carried out by RISC, the RNA-induced
silencing complex. RISC contains two signature components, small interfering RNAs …
silencing complex. RISC contains two signature components, small interfering RNAs …
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes
T Tadokoro, S Kanaya - The FEBS journal, 2009 - Wiley Online Library
The prokaryotic genomes, for which complete nucleotide sequences are available, always
contain at least one RNase H gene, indicating that RNase H is ubiquitous in all prokaryotic …
contain at least one RNase H gene, indicating that RNase H is ubiquitous in all prokaryotic …
Purified Argonaute2 and an siRNA form recombinant human RISC
FV Rivas, NH Tolia, JJ Song, JP Aragon, J Liu… - Nature structural & …, 2005 - nature.com
Genetic, biochemical and structural studies have implicated Argonaute proteins as the
catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human …
catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human …
Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutieres syndrome and mimic congenital viral brain infection
YJ Crow, A Leitch, BE Hayward, A Garner, R Parmar… - Nature …, 2006 - nature.com
Aicardi-Goutières syndrome (AGS) is an autosomal recessive neurological disorder, the
clinical and immunological features of which parallel those of congenital viral infection. Here …
clinical and immunological features of which parallel those of congenital viral infection. Here …
hu. MAP 2.0: integration of over 15,000 proteomic experiments builds a global compendium of human multiprotein assemblies
A general principle of biology is the self‐assembly of proteins into functional complexes.
Characterizing their composition is, therefore, required for our understanding of cellular …
Characterizing their composition is, therefore, required for our understanding of cellular …
Retroviral integrase superfamily: the structural perspective
M Nowotny - EMBO reports, 2009 - embopress.org
The retroviral integrase superfamily (RISF) comprises numerous important nucleic acid‐
processing enzymes, including transposases, integrases and various nucleases. These …
processing enzymes, including transposases, integrases and various nucleases. These …
Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts
B Rydberg, J Game - … of the National Academy of Sciences, 2002 - National Acad Sciences
Misincorporated ribonucleotides in DNA will cause DNA backbone distortion and may be
targeted by DNA repair enzymes. Using double-stranded oligonucleotide probes containing …
targeted by DNA repair enzymes. Using double-stranded oligonucleotide probes containing …
Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
A Carabias, A Fuglsang, P Temperini, T Pape… - Nature …, 2021 - nature.com
CRISPR-Cas12j is a recently identified family of miniaturized RNA-guided endonucleases
from phages. These ribonucleoproteins provide a compact scaffold gathering all key …
from phages. These ribonucleoproteins provide a compact scaffold gathering all key …
PCNA directs type 2 RNase H activity on DNA replication and repair substrates
Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in
eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides …
eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides …