Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

K Belhaj, A Chaparro-Garcia, S Kamoun, V Nekrasov - Plant methods, 2013‏ - Springer
Targeted genome engineering (also known as genome editing) has emerged as an
alternative to classical plant breeding and transgenic (GMO) methods to improve crop …

Immune signaling: receptor-like proteins make the difference

WRH Huang, MHAJ Joosten - Trends in Plant Science, 2024‏ - cell.com
To resist biotic attacks, plants have evolved a sophisticated, receptor-based immune system.
Cell-surface immune receptors, which are either receptor-like kinases (RLKs) or receptor …

NLR immune receptor–nanobody fusions confer plant disease resistance

J Kourelis, C Marchal, A Posbeyikian, A Harant… - Science, 2023‏ - science.org
Plant pathogens cause recurrent epidemics, threatening crop yield and global food security.
Efforts to retool the plant immune system have been limited to modifying natural components …

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

V Nekrasov, B Staskawicz, D Weigel, JDG Jones… - Nature …, 2013‏ - nature.com
To the Editor: Sustainable intensification of crop production is essential to ensure food
demand is matched by supply as the human population continues to increase1. This will …

TALEN and CRISPR/Cas genome editing systems: tools of discovery

AA Nemudryi, KR Valetdinova… - Acta Naturae …, 2014‏ - cyberleninka.ru
Precise studies of plant, animal and human genomes enable remarkable opportunities of
obtained data application in biotechnology and medicine. However, knowing nucleotide …

Retracted: An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus

O Voinnet, S Rivas, P Mestre, D Baulcombe - The plant journal, 2003‏ - Wiley Online Library
Transient gene expression is a fast, flexible and reproducible approach to high‐level
expression of useful proteins. In plants, recombinant strains of Agrobacterium tumefaciens …

Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing

R De Jonge, H Peter van Esse… - Proceedings of the …, 2012‏ - pnas.org
Fungal plant pathogens secrete effector molecules to establish disease on their hosts, and
plants in turn use immune receptors to try to intercept these effectors. The tomato immune …

A Draft Genome Sequence of Nicotiana benthamiana to Enhance Molecular Plant-Microbe Biology Research

A Bombarely, HG Rosli, J Vrebalov… - Molecular Plant …, 2012‏ - Am Phytopath Society
Nicotiana benthamiana is a widely used model plant species for the study of fundamental
questions in molecular plant-microbe interactions and other areas of plant biology. This …

Transient plant transformation mediated by Agrobacterium tumefaciens: Principles, methods and applications

P Krenek, O Samajova, I Luptovciak… - Biotechnology …, 2015‏ - Elsevier
Agrobacterium tumefaciens is widely used as a versatile tool for development of stably
transformed model plants and crops. However, the development of Agrobacterium based …

A Functional Genomics Approach Identifies Candidate Effectors from the Aphid Species Myzus persicae (Green Peach Aphid)

JIB Bos, D Prince, M Pitino, ME Maffei, J Win… - PLoS …, 2010‏ - journals.plos.org
Aphids are amongst the most devastating sap-feeding insects of plants. Like most plant
parasites, aphids require intimate associations with their host plants to gain access to …