It has long been axiomatic that a protein's structure determines its function. Intrinsically
disordered proteins (IDPs) and disordered protein regions (IDRs) defy this structure …

The intracellular milieu is complex, heterogeneous and crowded—an environment vastly
different from dilute solutions in which most biophysical studies are performed. The crowded …

A longstanding question in molecular biology is the extent to which the behavior of
macromolecules observed in vitro accurately reflects their behavior in vivo. A number of …

Despite increased attention, little is known about how the crowded intracellular environment
affects basic phenomena like protein diffusion. Here, we use NMR to quantify the rotational …

The interactions of clinically established anticancer Pt-based drugs with proteins play crucial
roles in Pt cellular uptake and biodistribution, as well as in determining side effects and …

Protein stability is usually studied in simple buffered solutions, but most proteins function
inside cells, where the heterogeneous and crowded environment presents a complex …

Although overexpression and 15N enrichment facilitate the observation of resonances from
disordered proteins in Escherichia coli, 15N enrichment alone is insufficient for detecting …

It is hard to imagine a more extreme contrast than that between the dilute solutions used for
in vitro studies of protein folding and the crowded, compartmentalized, sticky, spatially …