Deep convolutional neural networks for detecting secondary structures in protein density maps from cryo-electron microscopy

R Li, D Si, T Zeng, S Ji, J He - 2016 IEEE international …, 2016 - ieeexplore.ieee.org
The detection of secondary structure of proteins using three dimensional (3D) cryo-electron
microscopy (cryo-EM) images is still a challenging task when the spatial resolution of cryo …

Tracing beta strands using StrandTwister from cryo-EM density maps at medium resolutions

D Si, J He - Structure, 2014 - cell.com
Major secondary structure elements such as α helices and β sheets can be computationally
detected from cryoelectron microscopy (cryo-EM) density maps with medium resolutions of 5 …

Mathematical and Machine Learning Approaches for Classification of Protein Secondary Structure Elements from Coordinates

A Sekmen, K Al Nasr, B Bilgin, AB Koku, C Jones - Biomolecules, 2023 - mdpi.com
Determining Secondary Structure Elements (SSEs) for any protein is crucial as an
intermediate step for experimental tertiary structure determination. SSEs are identified using …

A unified framework for packing deformable and non-deformable subcellular structures in crowded cryo-electron tomogram simulation

S Liu, X Ban, X Zeng, F Zhao, Y Gao, W Wu, H Zhang… - BMC …, 2020 - Springer
Background Cryo-electron tomography is an important and powerful technique to explore
the structure, abundance, and location of ultrastructure in a near-native state. It contains …

Structural modeling from electron microscopy data

JR López‐Blanco, P Chacón - Wiley Interdisciplinary Reviews …, 2015 - Wiley Online Library
Cryo‐electron microscopy is a powerful technique for the determination of three‐
dimensional (3D) structures of macromolecular machines, as it provides functional …

Intensity-based skeletonization of CryoEM gray-scale images using a true segmentation-free algorithm

K Al Nasr, C Liu, M Rwebangira… - … /ACM transactions on …, 2013 - ieeexplore.ieee.org
Cryo-electron microscopy is an experimental technique that is able to produce 3D gray-
scale images of protein molecules. In contrast to other experimental techniques, cryo …

Exploratory studies detecting secondary structures in medium resolution 3D Cryo-EM images using deep convolutional neural networks

D Haslam, T Zeng, R Li, J He - Proceedings of the 2018 ACM …, 2018 - dl.acm.org
Cryo-electron microscopy (cryo-EM) is an emerging biophysical technique for structural
determination of protein complexes. However, accurate detection of secondary structures is …

Constrained cyclic coordinate descent for cryo-EM images at medium resolutions: beyond the protein loop closure problem

K Al Nasr, J He - Robotica, 2016 - cambridge.org
The cyclic coordinate descent (CCD) method is a popular loop closure method in protein
structure modeling. It is a robotics algorithm originally developed for inverse kinematic …

Comparing an atomic model or structure to a corresponding cryo-electron microscopy image at the central axis of a helix

S Zeil, J Kovacs, W Wriggers, J He - Journal of Computational …, 2017 - liebertpub.com
Three-dimensional density maps of biological specimens from cryo-electron microscopy
(cryo-EM) can be interpreted in the form of atomic models that are modeled into the density …

Analytical approaches to improve accuracy in solving the protein topology problem

K Al Nasr, F Yousef, R Jebril, C Jones - Molecules, 2018 - mdpi.com
To take advantage of recent advances in genomics and proteomics it is critical that the three-
dimensional physical structure of biological macromolecules be determined. Cryo-Electron …