Bridging structural and cell biology with cryo-electron microscopy
E Nogales, J Mahamid - Nature, 2024 - nature.com
Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …
structural knowledge about the macromolecules involved. For example, deciphering the …
Impact of novel detergents on membrane protein studies
Detergents are widely used for membrane protein solubilization, purification, and structural
analysis. Over the past two decades, much effort has been made to design novel detergents …
analysis. Over the past two decades, much effort has been made to design novel detergents …
Membranes under the magnetic lens: a dive into the diverse world of membrane protein structures using Cryo-EM
SJ Piper, RM Johnson, D Wootten, PM Sexton - Chemical reviews, 2022 - ACS Publications
Membrane proteins are highly diverse in both structure and function and can, therefore,
present different challenges for structure determination. They are biologically important for …
present different challenges for structure determination. They are biologically important for …
An evolving view of complex II—noncanonical complexes, megacomplexes, respiration, signaling, and beyond
TM Iverson, PK Singh, G Cecchini - Journal of Biological Chemistry, 2023 - jbc.org
Mitochondrial complex II is traditionally studied for its participation in two key respiratory
processes: the electron transport chain and the Krebs cycle. There is now a rich body of …
processes: the electron transport chain and the Krebs cycle. There is now a rich body of …
Single molecule protein segments sequencing by a plasmonic nanopore
J Zhou, Q Lan, W Li, LN Ji, K Wang, XH **a - Nano Letters, 2023 - ACS Publications
Obtaining sequential and conformational information on proteins is vital to understand their
functions. Although the nanopore-based electrical detection can sense single molecule (SM) …
functions. Although the nanopore-based electrical detection can sense single molecule (SM) …
Approaches to heterogeneity in native mass spectrometry
AD Rolland, JS Prell - Chemical reviews, 2021 - ACS Publications
Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …
composition, and stoichiometry of biomolecules and their complexes from solution after they …
Characterizing endogenous protein complexes with biological mass spectrometry
R Rogawski, M Sharon - Chemical reviews, 2021 - ACS Publications
Biological mass spectrometry (MS) encompasses a range of methods for characterizing
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …
[HTML][HTML] Overview of membrane protein sample preparation for single-particle cryo-electron microscopy analysis
C Vénien-Bryan, CAH Fernandes - International journal of molecular …, 2023 - mdpi.com
Single-particle cryo-electron microscopy (cryo-EM SPA) has recently emerged as an
exceptionally well-suited technique for determining the structure of membrane proteins …
exceptionally well-suited technique for determining the structure of membrane proteins …
Lysate-based pipeline to characterize microtubule-associated proteins uncovers unique microtubule behaviours
AS Jijumon, S Bodakuntla, M Genova, M Bangera… - Nature cell …, 2022 - nature.com
The microtubule cytoskeleton forms complex macromolecular assemblies with a range of
microtubule-associated proteins (MAPs) that have fundamental roles in cell architecture …
microtubule-associated proteins (MAPs) that have fundamental roles in cell architecture …
Cryo-EM structure of TMEM63C suggests it functions as a monomer
Y Qin, D Yu, D Wu, J Dong, WT Li, C Ye… - Nature …, 2023 - nature.com
The TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are
preferentially activated by hypo-osmolality, have been implicated in various physiological …
preferentially activated by hypo-osmolality, have been implicated in various physiological …