Coming of age: ten years of next-generation sequencing technologies
Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …
been made in genome sequencing technologies, which has led to a decreased cost per …
Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
We present two standards developed by the Genomic Standards Consortium (GSC) for
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …
[HTML][HTML] The sequence of sequencers: The history of sequencing DNA
Determining the order of nucleic acid residues in biological samples is an integral
component of a wide variety of research applications. Over the last fifty years large numbers …
component of a wide variety of research applications. Over the last fifty years large numbers …
DNA sequencing at 40: past, present and future
This review commemorates the 40th anniversary of DNA sequencing, a period in which we
have already witnessed multiple technological revolutions and a growth in scale from a few …
have already witnessed multiple technological revolutions and a growth in scale from a few …
High-throughput sequencing technologies
JA Reuter, DV Spacek, MP Snyder - Molecular cell, 2015 - cell.com
The human genome sequence has profoundly altered our understanding of biology, human
diversity, and disease. The path from the first draft sequence to our nascent era of personal …
diversity, and disease. The path from the first draft sequence to our nascent era of personal …
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
Sequencing technologies—the next generation
ML Metzker - Nature reviews genetics, 2010 - nature.com
Demand has never been greater for revolutionary technologies that deliver fast, inexpensive
and accurate genome information. This challenge has catalysed the development of next …
and accurate genome information. This challenge has catalysed the development of next …
Next-generation DNA sequencing
J Shendure, H Ji - Nature biotechnology, 2008 - nature.com
DNA sequence represents a single format onto which a broad range of biological
phenomena can be projected for high-throughput data collection. Over the past three years …
phenomena can be projected for high-throughput data collection. Over the past three years …
Real-time DNA sequencing from single polymerase molecules
J Eid, A Fehr, J Gray, K Luong, J Lyle, G Otto, P Peluso… - Science, 2009 - science.org
We present single-molecule, real-time sequencing data obtained from a DNA polymerase
performing uninterrupted template-directed synthesis using four distinguishable …
performing uninterrupted template-directed synthesis using four distinguishable …
Accurate whole human genome sequencing using reversible terminator chemistry
DR Bentley, S Balasubramanian, HP Swerdlow… - nature, 2008 - nature.com
DNA sequence information underpins genetic research, enabling discoveries of important
biological or medical benefit. Sequencing projects have traditionally used long (400–800 …
biological or medical benefit. Sequencing projects have traditionally used long (400–800 …