Visualization of omics data for systems biology

N Gehlenborg, SI O'donoghue, NS Baliga… - Nature …, 2010 - nature.com
High-throughput studies of biological systems are rapidly accumulating a wealth of'omics'-
scale data. Visualization is a key aspect of both the analysis and understanding of these …

RNA-Seq technology and its application in fish transcriptomics

X Qian, Y Ba, Q Zhuang, G Zhong - Omics: a journal of integrative …, 2014 - liebertpub.com
High-throughput sequencing technologies, also known as next-generation sequencing
(NGS) technologies, have revolutionized the way that genomic research is advancing. In …

The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data

A McKenna, M Hanna, E Banks, A Sivachenko… - Genome …, 2010 - genome.cshlp.org
Next-generation DNA sequencing (NGS) projects, such as the 1000 Genomes Project, are
already revolutionizing our understanding of genetic variation among individuals. However …

NGS QC Toolkit: a toolkit for quality control of next generation sequencing data

RK Patel, M Jain - PloS one, 2012 - journals.plos.org
Next generation sequencing (NGS) technologies provide a high-throughput means to
generate large amount of sequence data. However, quality control (QC) of sequence data …

Software for computing and annotating genomic ranges

M Lawrence, W Huber, H Pagès… - PLoS computational …, 2013 - journals.plos.org
We describe Bioconductor infrastructure for representing and computing on annotated
genomic ranges and integrating genomic data with the statistical computing features of R …

MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices

CS McGinnis, DM Patterson, J Winkler, DN Conrad… - Nature …, 2019 - nature.com
Sample multiplexing facilitates scRNA-seq by reducing costs and identifying artifacts such
as cell doublets. However, universal and scalable sample barcoding strategies have not …

Comprehensive genomic analysis of malignant pleural mesothelioma identifies recurrent mutations, gene fusions and splicing alterations

R Bueno, EW Stawiski, LD Goldstein, S Durinck… - Nature …, 2016 - nature.com
Abstract We analyzed transcriptomes (n= 211), whole exomes (n= 99) and targeted exomes
(n= 103) from 216 malignant pleural mesothelioma (MPM) tumors. Using RNA-seq data, we …

Count-based differential expression analysis of RNA sequencing data using R and Bioconductor

S Anders, DJ McCarthy, Y Chen, M Okoniewski… - Nature protocols, 2013 - nature.com
RNA sequencing (RNA-seq) has been rapidly adopted for the profiling of transcriptomes in
many areas of biology, including studies into gene regulation, development and disease. Of …

Genome-wide CRISPR screens in primary human T cells reveal key regulators of immune function

E Shifrut, J Carnevale, V Tobin, TL Roth, JM Woo… - Cell, 2018 - cell.com
Human T cells are central effectors of immunity and cancer immunotherapy. CRISPR-based
functional studies in T cells could prioritize novel targets for drug development and improve …

Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer

CM Rudin, S Durinck, EW Stawiski, JT Poirier… - Nature …, 2012 - nature.com
Small-cell lung cancer (SCLC) is an exceptionally aggressive disease with poor prognosis.
Here, we obtained exome, transcriptome and copy-number alteration data from …