The interaction of cells within their microenvironmental niche is fundamental to cell
migration, positioning, growth and differentiation in order to form and maintain complex …

The aim of this article is to review the basic principles of two-photon excitation fluorescence
(2PEF) microscopy and to compare the advantages and disadvantages of 2PEF imaging to …

Cancer cell invasion is an adaptive process based on cell-intrinsic properties to migrate
individually or collectively, and their adaptation to encountered tissue structure acting as …

Lipid bodies have an important role in energy storage and lipid regulation. Here we show
that lipid bodies are a major source of contrast in third-harmonic generation (THG) …

Quantifying cell behaviors in animal early embryogenesis remains a challenging issue
requiring in toto imaging and automated image analysis. We designed a framework for …

The ability to visualize neurons inside living brain tissue is a fundamental requirement in
neuroscience and neurosurgery. Especially the development of a noninvasive probe of …

Second-harmonic generation (SHG) microscopy began to emerge as a high-resolution
optical imaging modality in the late 1990s, about 10 years after two-photon excited …

Higher harmonic generation microscopy (HHGM), combining both second-and third-
harmonic generation (SHG and THG) modalities, is a new paradigm for in vivo noninvasive …

Interfaces provide the structural basis of essential bone functions. In the hierarchical
structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales …

Light-induced toxicity is a fundamental bottleneck in microscopic imaging of live embryos. In
this article, after a review of photodamage mechanisms in cells and tissues, we assess …