Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Abstract 3D printing alias additive manufacturing can transform 3D virtual models created by
computer-aided design (CAD) into physical 3D objects in a layer-by-layer manner …

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Spectrally distinct fluorophores are desired for multiplexed bioimaging. In particular,
monitoring biological processes in living mammals needs fluorophores that operate in the …

Making inferences about the computations performed by neuronal circuits from synapse-
level connectivity maps is an emerging opportunity in neuroscience. The mushroom body …

Dopamine is a prototypical neuromodulator that controls circuit function through G protein-
coupled receptor signalling. Neuromodulators are volume transmitters, with release followed …

Tissue clearing of gross anatomical samples was first described more than a century ago
and has only recently found widespread use in the field of microscopy. This renaissance has …

Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Mitochondria are key determinants of cellular health. However, the functional role of
mitochondria varies from cell to cell depending on the relative demands for energy …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …