The SpyCatcher-SpyTag system was developed seven years ago as a method for protein
ligation. It is based on a modified domain from a Streptococcus pyogenes surface protein …

Cancer represents a serious concern for human life and health. Due to drug resistance and
the easy metastasis of tumors, there is urgent need to develop new cancer treatment …

Autotransporters are a superfamily of proteins that use the type V secretion pathway for their
delivery to the surface of Gram-negative bacteria. At first glance, autotransporters look to …

Gram‐negative bacteria are surrounded by two membranes, called the outer and inner
membranes. The space between these membranes, the periplasm, is spanned by a …

Nanobodies (Nbs) are the smallest functional antibody fragments known in nature and have
multiple applications in biomedicine or environmental monitoring. Nbs are derived from the …

The two membranes of Gram-negative bacteria contain protein machines that have a
general function in their assembly. To interact with the extra-cellular milieu, Gram-negatives …

Autotransporters are a superfamily of virulence factors produced by Gram-negative bacteria
consisting of a large N-terminal extracellular domain (“passenger domain”) and a C-terminal …

Outer membrane vesicles (OMVs) are spherical nanoparticles that naturally shed from Gram-
negative bacteria. They are rich in immunostimulatory proteins and lipopolysaccharide but …

Gram-negative bacteria assemble many proteins into the inner and outer membranes and
export a large number of proteins to the periplasm or to the extracellular medium. During the …

Autotransporters are the core component of a molecular nano-machine that delivers cargo
proteins across the outer membrane of Gram-negative bacteria. Part of the type V secretion …