Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of
molecular dynamics, and it has been widely applied in diverse fields, eg, biomedicine …

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Enhancer-binding pluripotency regulators (Sox2 and Oct4) play a seminal role in embryonic
stem (ES) cell-specific gene regulation. Here, we combine in vivo and in vitro single …

The principle of microscopic reversibility says that, in equilibrium, two-time cross-correlations
are symmetric under the exchange of observables. Thus, the asymmetry of cross …

Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

Providing much-needed information on fluorescence spectroscopy and microscopy, this
ready reference covers detection techniques, data registration, and the use of spectroscopic …

The RNA-guided CRISPR-associated protein Cas9 is used for genome editing,
transcriptional modulation, and live-cell imaging. Cas9-guide RNA complexes recognize …

Fluorescence correlation spectroscopy (FCS) is an experimental technique using statistical
analysis of the fluctuations of fluorescence in a system in order to decipher dynamic …

In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …

Single-molecule biophysics has transformed our understanding of biology, but also of the
physics of life. More exotic than simple soft matter, biomatter lives far from thermal …