Transfectomas provide novel chimeric antibodies

SL Morrison - Science, 1985 - science.org
Methods have been developed to transfect immunoglobulin genes into lymphoid cells. The
transfected genes are faithfully expressed, and assembly can occur both between the …

Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor β

GGJM Kuiper, JG Lemmen, BO Carlsson… - …, 1998 - academic.oup.com
The rat, mouse and human estrogen receptor (ER) exists as two subtypes, ERα and ERβ,
which differ in the C-terminal ligand-binding domain and in the N-terminal transactivation …

Chimeric human antibody molecules: mouse antigen-binding domains with human constant region domains.

SL Morrison, MJ Johnson… - Proceedings of the …, 1984 - National Acad Sciences
We have created mouse-human antibody molecules of defined antigen-binding specificity
by taking the variable region genes of a mouse antibody-producing myeloma cell line with …

Large-scale transfection of mammalian cells for the fast production of recombinant protein

PL Pham, A Kamen, Y Durocher - Molecular biotechnology, 2006 - Springer
Recombinant proteins (r-proteins) are increasingly important in fundamental research and
for clinical applications. As many of these r-proteins are of human or animal origin, cultivated …

Electroporation for the efficient transfection of mammalian cells with DNA

G Chu, H Hayakawa, P Berg - Nucleic acids research, 1987 - academic.oup.com
A simple and reproducible procedure for the introduction of DNA into mammalian cells by
electroporation is described. The parameters involving the cells, the DNA, and the electric …

A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells.

J Yates, N Warren, D Reisman… - Proceedings of the …, 1984 - National Acad Sciences
The Epstein-Barr viral (EBV) genome of approximately equal to 170 kilobase pairs (kbp) is
maintained as a plasmid in human B lymphoblasts transformed by the virus. We have …

Expression of CYP3A in the human liver—evidence that the shift between CYP3A7 and CYP3A4 occurs immediately after birth

D Lacroix, M Sonnier, A Moncion… - European Journal of …, 1997 - Wiley Online Library
CYP3A isoforms are responsible for the biotransformation of a wide variety of exogenous
chemicals and endogenous steroids in human tissues. Two members of the CYP3A …

High efficiency polyoma DNA transfection of chloroquine treated cells

H Luthman, G Magnusson - Nucleic acids research, 1983 - academic.oup.com
Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection
increase the fraction of cells expressing viral functions. The effect has been observed after …

Cell therapy and neural stimulation for cardiac repair

I Libbus, J Ross, SD Girouard - US Patent 7,548,780, 2009 - Google Patents
US7548780B2 - Cell therapy and neural stimulation for cardiac repair - Google Patents
US7548780B2 - Cell therapy and neural stimulation for cardiac repair - Google Patents Cell …

Synthesis, processing, and secretion of recombinant human factor VIII expressed in mammalian cells.

RJ Kaufman, LC Wasley, AJ Dorner - Journal of Biological Chemistry, 1988 - Elsevier
The synthesis, processing, and secretion of factor VIII expressed from heterologous genes
introduced into Chinese hamster ovary cells has been studied. The results show factor VIII to …