The intriguing mitoNEET: functional and spectroscopic properties of a unique [2Fe-2S] cluster coordination geometry

F Camponeschi, M Piccioli, L Banci - Molecules, 2022 - mdpi.com
Despite the number of cellular and pathological mitoNEET-related processes, very few
details are known about the mechanism of action of the protein. The recently discovered …

[HTML][HTML] Aqueous stability and redox chemistry of synthetic [Fe4S4] clusters

V Waser, TR Ward - Coordination Chemistry Reviews, 2023 - Elsevier
Iron-sulfur proteins are ubiquitous in nature, acting as electron carriers and catalysts. Hence,
a plethora of synthetic analogs has been prepared to serve as active site models. However …

Relaxation-based NMR assignment: Spotlights on ligand binding sites in human CISD3

D Grifagni, JM Silva, F Cantini, M Piccioli… - Journal of Inorganic …, 2023 - Elsevier
CISD3 is a mitochondrial protein belonging to the NEET proteins family, bearing two [Fe 2 S
2] clusters coordinated by CDGSH domains. At variance with the other proteins of the NEET …

Paramagnetic nuclear magnetic resonance: The Toolkit

L Querci, L Fiorucci, E Ravera, M Piccioli - Inorganics, 2023 - mdpi.com
Nuclear Magnetic Resonance (NMR) spectroscopy is the ideal tool to address the structure,
reactivity and dynamics of both inorganic and biological substances. The knowledge of …

NMR of Paramagnetic Proteins: 13C Derived Paramagnetic Relaxation Enhancements Are an Additional Source of Structural Information in Solution

L Querci, IB Trindade, M Invernici, JM Silva, F Cantini… - Magnetochemistry, 2023 - mdpi.com
In paramagnetic metalloproteins, longitudinal relaxation rates of 13C′ and 13Cα nuclei can
be measured using 13C detected experiments and converted into electron spin-nuclear spin …

Electron transfer in biological systems

HM Marques - JBIC Journal of Biological Inorganic Chemistry, 2024 - Springer
Examples of how metalloproteins feature in electron transfer processes in biological systems
are reviewed. Attention is focused on the electron transport chains of cellular respiration and …

[HTML][HTML] Regulations of mitoNEET by the key redox homeostasis molecule glutathione

C Mons, M Salameh, T Botzanowski… - Journal of Inorganic …, 2024 - Elsevier
Human mitoNEET (mNT) and CISD2 are two NEET proteins characterized by an atypical
[2Fe-2S] cluster coordination involving three cysteines and one histidine. They act as redox …

1H, 13C and 15N assignment of the human mitochondrial paramagnetic iron–sulfur protein CISD3

JM Silva, D Grifagni, F Cantini, M Piccioli - Biomolecular NMR …, 2023 - Springer
CISD3 is a mitochondrial protein that contains two [2Fe–2S] clusters. This protein is
overexpressed in some types of cancer, so it has emerged as a potential drug target. A …

Fast 2D NMR to Investigate Dynamic Events in Biomolecules

F Bruno, E Luchinat, K Kazimierczuk, E Ravera - 2023 - books.rsc.org
Biological macromolecules (proteins, nucleic acids and polysaccharides) are highly
complex in terms of not only their structure but also dynamical and kinetic properties …