Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection

D Mayo-Muñoz, LM Smith, C Garcia-Doval, LM Malone… - Molecular cell, 2022 - cell.com
Bacteria have diverse defenses against phages. In response, jumbo phages evade multiple
DNA-targeting defenses by protecting their DNA inside a nucleus-like structure. We …

The intricate organizational strategy of nucleus-forming phages

A Prichard, J Pogliano - Current Opinion in Microbiology, 2024 - Elsevier
Nucleus-forming phages (chimalliviruses) encode numerous genes responsible for creating
intricate structures for viral replication. Research on this newly appreciated family of phages …

[HTML][HTML] Identifying the core genome of the nucleus-forming bacteriophage family and characterization of Erwinia phage RAY

A Prichard, J Lee, TG Laughlin, A Lee, KP Thomas… - Cell reports, 2023 - cell.com
We recently discovered that some bacteriophages establish a nucleus-like replication
compartment (phage nucleus), but the core genes that define nucleus-based phage …

Nucleus-forming vibriophage cocktail reduces shrimp mortality in the presence of pathogenic bacteria

K Thammatinna, A Sinprasertporn, A Naknaen… - Scientific Reports, 2023 - nature.com
The global aquaculture industry has suffered significant losses due to the outbreak of Acute
Hepatopancreatic Necrosis Disease (AHPND) caused by Vibrio parahaemolyticus. Since …

A phage nucleus-associated RNA-binding protein is required for jumbo phage infection

E Enustun, EG Armbruster, J Lee, S Zhang… - Nucleic Acids …, 2024 - academic.oup.com
Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae
assemble a nucleus-like compartment bounded by a protein shell that protects the …

Microbial evolution through horizontal gene transfer by mobile genetic elements

M Tokuda, M Shintani - Microbial Biotechnology, 2024 - Wiley Online Library
Mobile genetic elements (MGEs) are crucial for horizontal gene transfer (HGT) in bacteria
and facilitate their rapid evolution and adaptation. MGEs include plasmids, integrative and …

[HTML][HTML] Sequential membrane-and protein-bound organelles compartmentalize genomes during phage infection

EG Armbruster, J Lee, J Hutchings, AR VanderWal… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Eukaryotic viruses assemble compartments required for genome replication, but no such
organelles are known to be essential for prokaryotic viruses. Bacteriophages of the family …

An essential and highly selective protein import pathway encoded by nucleus-forming phage

CJ Morgan, E Enustun, EG Armbruster… - Proceedings of the …, 2024 - National Acad Sciences
Targeting proteins to specific subcellular destinations is essential in prokaryotes,
eukaryotes, and the viruses that infect them. Chimalliviridae phages encapsulate their …

Jumbo phages are active against extensively drug-resistant eyedrop-associated Pseudomonas aeruginosa infections

AG Cobián Güemes, P Ghatbale… - Antimicrobial Agents …, 2023 - Am Soc Microbiol
Antibiotic-resistant bacteria present an emerging challenge to human health. Their
prevalence has been increasing across the globe due in part to the liberal use of antibiotics …

Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells

M Lu, Z Lee, WS Hu - Biotechnology Progress, 2024 - Wiley Online Library
Recombinant adeno‐associated virus (rAAV) is among the most commonly used vectors for
gene therapy. It is commonly produced by transfection of HEK293 cells with three plasmids …