Cell-free gene expression
D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …
specific applications in protein synthesis and labelling for structural biology and proteomics …
CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
How to measure and evaluate binding affinities
Quantitative measurements of biomolecule associations are central to biological
understanding and are needed to build and test predictive and mechanistic models. Given …
understanding and are needed to build and test predictive and mechanistic models. Given …
Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosis
Antibacterial agents target the products of essential genes but rarely achieve complete
target inhibition. Thus, the all-or-none definition of essentiality afforded by traditional genetic …
target inhibition. Thus, the all-or-none definition of essentiality afforded by traditional genetic …
Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq
CRISPR-Cas genome editing induces targeted DNA damage but can also affect off-target
sites. Current off-target discovery methods work using purified DNA or specific cellular …
sites. Current off-target discovery methods work using purified DNA or specific cellular …
Increasing the specificity of CRISPR systems with engineered RNA secondary structures
CRISPR (clustered regularly interspaced short palindromic repeat) systems have been
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …
Single-cell lineages reveal the rates, routes, and drivers of metastasis in cancer xenografts
INTRODUCTION Cancer progression is fundamentally an evolutionary process, involving
multiple distinct steps from oncogenic transformation to metastatic dissemination to …
multiple distinct steps from oncogenic transformation to metastatic dissemination to …
Kinetic basis for DNA target specificity of CRISPR-Cas12a
Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …
applications in human health and disease. However, DNA cleavage at off-target sites that …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor
S Balderston, JJ Taulbee, E Celaya, K Fung… - Nature Biomedical …, 2021 - nature.com
Simple and fast methods for the detection of target genes with single-nucleotide specificity
could open up genetic research and diagnostics beyond laboratory settings. We recently …
could open up genetic research and diagnostics beyond laboratory settings. We recently …