Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

The promise of single-objective light-sheet microscopy is to combine the convenience of
standard single-objective microscopes with the speed, coverage, resolution and gentleness …

High-throughput methods for slide-free three-dimensional (3D) pathological analyses of
whole biopsies and surgical specimens offer the promise of modernizing traditional …

Since its initial demonstration in 2000, far-field super-resolution light microscopy has
undergone tremendous technological developments. In parallel, these developments have …

Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …

Due to the size and opacity of vertebrate brains, it has until now been impossible to
simultaneously record neuronal activity at cellular resolution across the entire adult brain. As …

Life emerges from thousands of biochemical processes occurring within a shared
intracellular environment. We have gained deep insights from in vitro reconstitution of …

Confocal microscopy remains a major workhorse in biomedical optical microscopy owing to
its reliability and flexibility in imaging various samples, but suffers from substantial point …

Histological analysis of biological tissues by mechanical sectioning is significantly time‐
consuming and error‐prone due to loss of important information during sample slicing. In the …