Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli

F Silva, JA Queiroz, FC Domingues - Biotechnology advances, 2012 - Elsevier
In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …

Current trends in separation of plasmid DNA vaccines: A review

A Ghanem, R Healey, FG Adly - Analytica chimica acta, 2013 - Elsevier
Plasmid DNA (pDNA)-based vaccines offer more rapid avenues for development and
production if compared to those of conventional virus-based vaccines. They do not rely on …

Separation of plasmid DNA topological forms, messenger RNA, and lipid nanoparticle aggregates using an ultrawide pore size exclusion chromatography column

A Goyon, S Tang, S Fekete, D Nguyen… - Analytical …, 2023 - ACS Publications
Health authorities have highlighted the need to determine oligonucleotide aggregates.
However, existing technologies have limitations that have prevented the reliable analysis of …

Bioreducible poly (2-ethyl-2-oxazoline)–PLA–PEI-SS triblock copolymer micelles for co-delivery of DNA minicircles and Doxorubicin

VM Gaspar, P Baril, EC Costa, D de Melo-Diogo… - Journal of controlled …, 2015 - Elsevier
The co-delivery of minicircle DNA (mcDNA) and small anti-cancer drugs via stimuli-sensitive
nanocarriers is a promising approach for combinatorial cancer therapy. However, the …

Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas

AP Nievergelt, DR Diener, A Bogdanova, T Brown… - Cell reports …, 2023 - cell.com
CRISPR-Cas genome engineering in the unicellular green algal model Chlamydomonas
reinhardtii has until now been primarily applied to targeted gene disruption, whereas …

Ribonucleic acid purification

R Martins, JA Queiroz, F Sousa - Journal of Chromatography A, 2014 - Elsevier
Research on RNA has led to many important biological discoveries and improvement of
therapeutic technologies. From basic to applied research, many procedures employ pure …

[HTML][HTML] Recent advances in chromatographic purification of plasmid DNA for gene therapy and DNA vaccines: A review

A Abdulrahman, A Ghanem - Analytica chimica acta, 2018 - Elsevier
The wide spread of infectious diseases have provoked the scientists to develop new types of
vaccines. Among the different types of vaccines, the recently discovered plasmid DNA …

Advances in chromatographic supports for pharmaceutical‐grade plasmid DNA purification

 Sousa, F Sousa, JA Queiroz - Journal of separation science, 2012 - Wiley Online Library
Chromatographic technology is undoubtedly one of the most diverse and powerful
purification methods for downstream process applications. The diversity and quantity of …

[HTML][HTML] Gene-activated materials in regenerative dentistry: narrative review of technology and study results

O Krasilnikova, A Yakimova, S Ivanov… - International Journal of …, 2023 - mdpi.com
Treatment of a wide variety of defects in the oral and maxillofacial regions requires the use
of innovative approaches to achieve best outcomes. One of the promising directions is the …

Analytical and Functional Characterization of Plasmid DNA Topological Forms and Multimers

DN Nguyen, P Ko, B Roper, G Console, Y Gao… - Analytical …, 2024 - ACS Publications
Plasmid DNA (pDNA) is an essential tool in genetic engineering that has gained prevalence
in cell and gene therapies. Plasmids exist as supercoiled (SC), open circular (OC), and …