Data‐independent acquisition mass spectrometry‐based proteomics and software tools: a glimpse in 2020

F Zhang, W Ge, G Ruan, X Cai, T Guo - Proteomics, 2020 - Wiley Online Library
This review provides a brief overview of the development of data‐independent acquisition
(DIA) mass spectrometry‐based proteomics and selected DIA data analysis tools. Various …

Bottom-up proteomics: advancements in sample preparation

VA Duong, H Lee - International journal of molecular sciences, 2023 - mdpi.com
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …

Itaconate and itaconate derivatives target JAK1 to suppress alternative activation of macrophages

MC Runtsch, S Angiari, A Hooftman, R Wadhwa… - Cell metabolism, 2022 - cell.com
The Krebs cycle-derived metabolite itaconate and its derivatives suppress the inflammatory
response in pro-inflammatory" M1" macrophages. However, alternatively activated" M2" …

[HTML][HTML] Mucus sialylation determines intestinal host-commensal homeostasis

Y Yao, G Kim, S Shafer, Z Chen, S Kubo, Y Ji, J Luo… - Cell, 2022 - cell.com
Intestinal mucus forms the first line of defense against bacterial invasion while providing
nutrition to support microbial symbiosis. How the host controls mucus barrier integrity and …

HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells

H Yu, S Lu, K Gasior, D Singh, S Vazquez-Sanchez… - Science, 2021 - science.org
INTRODUCTION Aggregation of the RNA binding protein TDP-43 (TAR DNA-binding protein
43) is a common pathological hallmark shared by several age-related neurodegenerative …

AlphaPeptDeep: a modular deep learning framework to predict peptide properties for proteomics

WF Zeng, XX Zhou, S Willems, C Ammar… - Nature …, 2022 - nature.com
Abstract Machine learning and in particular deep learning (DL) are increasingly important in
mass spectrometry (MS)-based proteomics. Recent DL models can predict the retention …

A high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides

ZL Chen, JM Meng, Y Cao, JL Yin, RQ Fang… - Nature …, 2019 - nature.com
We describe pLink 2, a search engine with higher speed and reliability for proteome-scale
identification of cross-linked peptides. With a two-stage open search strategy facilitated by …

Precise, fast and comprehensive analysis of intact glycopeptides and modified glycans with pGlyco3

WF Zeng, WQ Cao, MQ Liu, SM He, PY Yang - Nature Methods, 2021 - nature.com
Great advances have been made in mass spectrometric data interpretation for intact
glycopeptide analysis. However, accurate identification of intact glycopeptides and modified …

Identification of modified peptides using localization-aware open search

F Yu, GC Teo, AT Kong, SE Haynes… - Nature …, 2020 - nature.com
Identification of post-translationally or chemically modified peptides in mass spectrometry-
based proteomics experiments is a crucial yet challenging task. We have recently introduced …

The cytosolic thiol peroxidase PRXIIB is an intracellular sensor for H2O2 that regulates plant immunity through a redox relay

G Bi, M Hu, L Fu, X Zhang, J Zuo, J Li, J Yang, JM Zhou - Nature plants, 2022 - nature.com
Rapid production of H2O2 is a hallmark of plant responses to diverse pathogens and plays a
crucial role in signalling downstream of various receptors that perceive immunogenic …